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利用实时定量PCR检测儿童急性淋巴细胞白血病的E2A-PBX1融合基因 被引量：1

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摘要 3%～5%的儿童急性淋巴细胞白血病（ALL）和5%的成人ALL患者,携带t（1;19）（q23;p13）,该易位形成E2A-PBX1（Immunoglobulin Enhancer Binding Factor-Pre-B Leukemia）融合基因.实时定量PCR（RQ-PCR）是近年来得到广泛应用的基因定量技术,已成为PCR更新换代的方法.我们采用RQ-PCR方法成功检测了27例ALL患儿初诊时E2APBX1融合基因表达水平,并定量检测了其中9例患儿诱导缓解治疗结束时的微量残留病（MRD）水平.
作者 高超 李志刚 赵玮 吴敏媛 张永红 石慧文 谢静 胡亚美
机构地区 首都医科大学附属北京儿童医院血液病中心
出处 《中华血液学杂志》 CAS CSCD 北大核心 2006年第5期346-347,共2页 Chinese Journal of Hematology
关键词 儿童急性淋巴细胞白血病 定量PCR检测 融合基因 实时定量 基因表达水平 Q-PCR方法 基因定量技术 诱导缓解治疗 微量残留病 定量检测
分类号 R733.71 [医药卫生—肿瘤] R512.62 [医药卫生—内科学]
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参考文献5

  • 1李志刚,吴敏媛,赵玮,李彬,杨静,朱平,胡亚美.多重RT-PCR方法同时检测29种白血病融合基因[J].中华血液学杂志,2003,24(5):256-258. 被引量：45
  • 2van Dongen JJ,Macintyre EA,Gabert JA,et al.Standardized RTPCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease.Report of the BIOMED-1 Concerted Action:investigation of minimal residual disease in acute leukemia.Leukemia,1999,13:1901-1928.
  • 3Gabert J,Beillard E,van der Velden VH,et al.Standardization and quality control studies of ‘ real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia-a Europe Against Cancer program.Leukemia,2003,17:2318-2357.
  • 4Beillard E,Pallisgaard N,van der Velden VH,et al.Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using ‘real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR)-a Europe Against Cancer Program.Leukemia,2003,17:2474-2486.
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二级参考文献1

共引文献44

同被引文献11

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  • 5Gabert J, Beillard E, van-der-Velden VII, et al. Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia-a Europe Against Cancer program. Leukemia,2003 ; 17:2318 - 2357.
  • 6Beillard E, Pallisgaard N, van-der-Velden VII, et al. Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time' quantitative reverse-transcrptase polymerase chain reaction (RQ-PCR)-a Europe Against Cancer Program. Leukemia,2003 ; 17 : 2474 - 2486.
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中华血液学杂志
2006年 第5期

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