摘要
目的用不同质量浓度2,4-D和外植体类型对刺五加体细胞胚胎诱导进行研究,为刺五加的资源保护和遗传工程提供理论依据。方法以刺五加的萌发3周幼苗和层积处理种子合子胚的胚根、下胚轴和子叶为外植体,考察不同激素对刺五加体细胞胚胎发生的影响。结果来自层积处理种子合子胚的外植体接种于附加0.5 mg/L2,4-D的MS或1/2MS培养基中体细胞胚胎诱导率(57.1%)和数量(3.3)最高。体胚在原培养基中即可发育成熟,转入降低2,4-D质量浓度的培养基后更有利于新体胚的产生和原有体胚的成熟。刺五加体胚的发育历程与合子胚相似。结论刺五加可以通过来自合子胚的外植体实现体细胞胚胎发生,体胚诱导率取决于2,4-D质量浓度和外植体的发育程度。
Objective To study somatic cell embryogenesis of Acanthopanax senticosus induced by different concentration of 2,4-D and type of explants, which provides theoretic evidence in protection of A. senticosus resources and genetic engineering. Methods Using 3-week seedlings and zygotic embryos （cotyledon, hypocotyls, and roots） of stratificated seeds as explants researches the effect of different hormones on somatic cell embryogenesis of A. senticosus. Results Explants of zygotic embryos of stratificated seeds cultured on MS and 1/2MS media containing 0. 5 mg/L 2,4-D generated the highest frequency （57.1%） and the most number （3.3） of somatic cell embryos, which can develop into maturation in the initial medium. But it is more beneficial to generate new somatic cell embryos and to develop primary somatic cell embryos into maturation when transferred into 2,4-D of decreased concentration. And the development process of somatic cell embryos of A. senticosus is similar to that of zygotic embryos. Conclusion Somatic embryogenesis of A. senticosus is realized by culturing explants of zygotic embryos and the inductive rate of somatic cell embryos is related to the concentration of 2,4-D and developmental stage of explants.
出处
《中草药》
CAS
CSCD
北大核心
2006年第5期769-772,共4页
Chinese Traditional and Herbal Drugs
基金
国家"十五"科技攻关子课题(2001BA510B0801)
关键词
刺五加
体细胞胚胎发生
2
4-D
Acanthopanax senticosus Harms
somatic cell embryogenesis
2,4-D
