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长时连续作业对大鼠下丘脑及背缝核orexin-A和受体的影响 被引量：5

Effects of long-term continuous operations on orexin-A and OX1R expression in the rat brain
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摘要 目的研究长时连续作业对大鼠促觉醒肽orexin- A及受体表达的影响。方法使用长时连续作业动物模型,采用免疫组织化学方法结合图像分析技术测定orexin -A及受体OX1R在下丘脑外侧区,背缝核的变化。结果经过长时连续作业训练后,跑台组与对照组相比,orexin- A阳性细胞在下丘脑外侧区、orexin- A阳性纤维在背缝核及OX1R在背缝核均显著减少。结论长时连续作业可造成促觉醒肽orexin- A和OX1R的表达降低。 Objective To investigate the effects of long-term continuous operations on orexin-A and its receptor （OX1R） expression in the lateral hypothalamic area （LHA） and dorsal raphe nucleus （DR） of a rat model. Methods Rats exercised for two circadian cycles using a treadmill protocol. Subsequently, immunohistochemical techniques combined with quantitative analysis of images techniques were performed to detect the expression of orexin-A and OX1R in rat LHA and DR. Results The number of orexin-A immunoreactive neurons in LHA, orexin-A immunoreactive fibers and OX1R in DR of operational animals was significantly decreased as compared with control group （P 〈 0. 01 ）. Conclusion Rat alertness activities are deleteriously influenced by long-term continuous operations, which may be related to the depressed expression of orexin-A and its receptor in the arousal systems.
作者 王臻 谌小维 张春青 樊宏孝 程赛宇 阮怀珍 胡志安
机构地区 第三军医大学基础医学部神经生物学教研室 第三军医大学基础医学部基础医学教学实验中心
出处 《第三军医大学学报》 CAS CSCD 北大核心 2006年第10期1046-1048,共3页 Journal of Third Military Medical University
基金 第三军医大学军事医学创新预研基金资助项目(XJYY200310)~~
关键词 长时连续作业 OREXIN-A 疲劳 觉醒 免疫组织化学 背缝核 下丘脑 long-term continuous operations orexin-A fatigue wakefulness immunohistochemistry
分类号 R338.27 [医药卫生—人体生理学]
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参考文献10

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二级参考文献31

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第三军医大学学报
2006年 第10期

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