摘要
为了探索我国北方汉族人群人类白细胞抗原－ＤＲ（ＨＬＡ－ＤＲ）及肿瘤坏死因子Ｂ（ＴＮＦＢ）等位基因多态性与系统性红斑狼疮（ＳＬＥ）的易感性关系，对１０６例健康人和４５例ＳＬＥ患者的ＨＬＡ－ＤＲ和对８０例健康人及４５例ＳＬＥ患者的ＴＮＦＢ等位基因，采用多聚酶链反应－限制性酶切片段长度多态性（ＰＣＲ－ＲＦＬＰ）法进行分析。结果显示：ＳＬＥ患者中频率显著升高的等位基因有ＤＲ２［Ｐ＜０．０５，相对危险性（ＲＲ）＝１．５６］及ＤＲ３（Ｐ＜０．０１，ＲＲ＝２．６９）。而ＤＲ５和对照相比呈相反结果（Ｐ＜０．０５，ＲＲ＝０．４３）。ＳＬＥ患者ＴＮＦＢ＊２等位基因频率显著增高（Ｐ＜０．０５，ＲＲ＝１．８４）。提示ＤＲ２、ＤＲ３、ＴＮＦＢ＊２可能是易感等位基因或易感等位基因标记，而ＤＲ５是一拮抗等位基因或拮抗等位基因标记。并研究了ＨＬＡ－ＤＲ、ＴＮＦＢ等位基因与患者血浆中Ｓ蛋白和补体５ｂ－９结合物的复合物（ＳＣ５ｂ－９）的水平和多种自身抗体及狼疮肾炎、狼疮肺炎、狼疮脑病等狼疮并发症之间的相关性，发现ＨＬＡ－ＤＲ２基因与狼疮肾炎的发生存在正相关（Ｐ＜０．０５，ＲＲ＝１．３２）。
InordertoexplorethealelicpolymorphismofHLA-DRandTNFBlociandsusceptibil-itytosystemiclupuserythematosus(SLE)intheHannationalityofnorthernChinawiththeaidofmethodsofpolymerasechainreaction/sequencespecificprimers(PCR/SSP)andpolymerasechainreac-tion-restrictionfragmentlengthpolymorphism(PCR-RFLP)respectively.Thefindingsfromacase-controlstudyon151bloodsamples(45fromthecasesand106fromthecontrols)indicatedthatthereweresignificantlyhigherfrequencyofDR2(P<0.05,RR=1.56)andDR3(P<0.01,RR=2.69),whichrepresentcandidatesusceptiblegenesorusefulmarkersforSLE.TheDR5aleleinthesamples(P<0.05,RR=0.43)mightbeanantagonisticorprotectivealele,oramarkerforsuchalele.ThefrequencyofTNFB*1andTNFB*2aleleswasinvestigatedin45SLEpatientsand80healthycon-trolsanditwasfoundthatthefrequencyofTNFB*2alelewassignificantlyhigherinthepatientgroup(P<0.05,RR=1.84).Itmightalsobeasuspicioussusceptiblealeleoramarkerforsuchal-lele.ThefrequencyofHLApolymorphismsinvariousclinical/immunologicalsubsetsofourpatientpopulationwasalsodetermined.ClinicalfindingsusedincludeplasmaSC5b-9level,SSA,SSB,Sm,RNP,ANAantibodies,andSLEcomplications(SLEnephritis,pneumonia&encephalopathy).ItturnedoutthattherewasapositiveassociationbetweenHLA-DR2aleleandSLEnephritis(P<0.05,RR=1.32).
出处
《中华内科杂志》
CAS
CSCD
北大核心
1996年第1期19-22,共4页
Chinese Journal of Internal Medicine
