摘要
研究了两种诱导分化剂星状孢子素（ｓｔａｕｒｏｓｐｏｒｉｎｅ）及金雀异黄素（ｇｅｎｉｓｔｅｉｎ）在诱导人早幼粒白血病细胞（ＨＬ－６０细胞）的过程中酪氨酸蛋白激酶（ＴＰＫ）及酪氨酸蛋白磷酸酶（ＰＴＰＰ）的活力变化。结果表明，两种分化诱导剂对胞浆及膜两部分的ＴＰＫ活力均有不同程度的抑制，而对这两部分的ＰＴＰＰ活力则有不同程度的激活作用，说明分化诱导剂可使ＨＬ－６０细胞内的酪氨酸的磷酸化水平提高，揭示了诱导分化与酪氨酸磷酸化及脱磷酸化之间的关系，为寻找新的抗癌药物提供了帮助。
The activities of tyrosine protein kinase (TPK) and phosphotyrosine protein phosphatase were studied while differentiation of human promyelocytic leukemia (HL-60) cells were induced by staurosporine and genistein.The results showed that TPK activities decreased and PTPP activities increased with obtainment of the differentiated state.It suggested that level of total tyrosine phosphorylation decreased during induced differentiation of HL-60 cells.The activities of TPK decreased after it was treated by staurosporine and genistein for 10 minutes respectively. The present study indicated that the protein phosphorylation and dephosphorylation associated with induced differentiation of tumor cells.
出处
《中国肿瘤临床》
CSCD
北大核心
1996年第2期84-88,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金
北京医科大学天然药物及仿生药物国家重点实验室资助
关键词
白血病
HL-60细胞
TPK
PTPP
星状孢子素
Induced differentiation Tyrosine protein kinase (TPK)Phosphotyrosine protein phosphatase (PTPP)Staurosporine Genistein
