摘要
目的探讨ESAT-6体外诱导的γ-干扰素释放反应(简称ESAT-6反应)在人免疫缺陷病毒(H IV)合并结核杆菌(TB)感染中的诊断价值。方法选择H IV(+)TB(+)患者78例,H IV(+)TB(-)患者60例,H IV(-)TB(+)患者30例,健康对照者20例,用全血测定法检测ESAT-6反应。结果ESAT-6反应在H IV(+)患者合并和非合并结核患者中有显著性差异(P<0.01),ESAT-6反应对H IV(+)TB(+)患者检出率明显高于PPD皮试、PPD诱导的γ-干扰素反应。结论我们建立的ESAT-6反应测定法简便、灵敏、特异性高,对H IV合并结核感染患者具有较好的诊断价值。
[Objective] To explore the value of interferon γ（IFN-γ） response induced by ESAT-6 in vitro in mycobacterium tuberculosis （TB） diagnosis among human immunodeficiency virus （HIV） seropositive patients. [Methods] 78 HIV（＋）TB（＋） patients,60 HIV （＋） TB （-） patients, 30 HIV （-） TB （＋） patients and 20 normal controls were selected. Heparinized whole blood was incubated at 37℃ for 16-20 hours after ESAT-6 was added. IFN-γ was detected by ELISA method. [Results] The occurrence rate of IFN-γ response induced by ESAT-6 in vitro was much higher than that of PPD skin test or PPD-induced IFN-γ response. There was a significant difference between the HIV （＋） TB （＋） group and the HIV （＋） TB （-） group compared to control groups （P〈0. 01）. [Conclusion] This work set up the method of ESAT-6-induced IFN-γ response in vitro. This method is simple, sensitive and specific. It has some value in diagnosis of HIV coinfected TB.
出处
《山东医药》
CAS
北大核心
2006年第16期16-17,共2页
Shandong Medical Journal
基金
河南省医学科技创新人才工程项目(2002112)
关键词
免疫缺陷病毒
分枝杆菌
结核
感染
干扰素Ⅱ型
immunodeficiency virus
mycobacterium, tuberculosis
infection
interferon type Ⅱ
