摘要
从太平洋深海底泥样品中富集、筛选得到50株锰抗性细菌,16S rDNA鉴定结果表明这50株细菌中含有40株芽孢杆菌、3株短杆菌、1株短状杆菌、1株考克氏菌、4株副球菌和1株食烷菌.50株中80%是芽孢杆菌,有6个种.90%以上为革兰氏阳性菌.短小芽孢杆菌(B.pum ilus)Mn12对Mn2+的耐受能力最好,最高的Mn2+耐受浓度是130 mmol/L.该菌在28℃以200 r/m in培养24 h后,能去除含锰(Ⅱ)10 mmol/L培养基中90%的锰(Ⅱ)(锰含量由高碘酸盐分光光度法测定).而Mn59在同样条件下,可耐受30 mmol/L锰(Ⅱ),可去除99%的锰(Ⅱ),具有抗性高、去除率高、速度快的优点,具有极大的应用前景.
50 manganese resistant bacterial stains have been obtained from four samples of Pacific deep sea sediments. Sequence analysis of 16S rDNA identifies that the 50 bacteria belong to Bacillus （40 isolates）, Brevibacterium （3 isolates）, Brachybacterium （1 isolates）, Kocuria （1 isolates）, Paracoccus （4 isolates） and Alcanivorax （1 isolates）. 80% of the 50 isolates belong to Bacillus which contain 6 species and 90% of the 50 isolates are Gram positive. Among them, Bacillus sp. Mn12 has the highest manganese resistance ability to 130 mmol/L Mn（Ⅱ） when it grows in LB broth at 28℃ and removes 90 percent of Mn^2＋ from the culture （10 mmol/L Mn（Ⅱ）） after 24 h sample incubation at 28℃ with shaking at 200 r/min. （Patassium periodate spectrophotometric method to deternine the manganese content）. Under the same condition, Mn59 can grow in the culture supplemented with 30 mmol/L Mn（Ⅱ） and remove 99% Mn（Ⅱ）. Its wider resistance range, higher removal rate and shorter cultivating time show its bright application prospect.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第B05期272-276,共5页
Journal of Xiamen University：Natural Science
基金
国家重点基础研究发展计划(973计划)(2004CB719601)
中国大洋协会资源加工利用项目(DY105-04-02-06)资助
关键词
深海细菌
锰抗性
重金属去除
deep sea bacteria
manganese resistance
heavy metal removal
