摘要
AIM： To determine whether gastric and enteric Helicobacter species are associated with pancreatic cancer. METHODS： Patients with exocrine pancreatic cancer （n = 40）, neuroendocrine cancer （n = 14）, multiple endocrine neoplasia type 1 （n = 8）, and chronic pan- creatitis （n = 5） were studied. Other benign pancreatic diseases （n = 10） and specimens of normal pancreas （n = 7） were included as controls. Pancreatic tissue specimens were analyzed by Helicobacter-specific PCR-assay and products were characterized by denaturing gradient electrophoresis and DNA-sequencing. From a subset of the pancreatic cancer patients, gastric and/or duodenal tissue as well as gallbladder and ductus choledochus tissue were analyzed. Gallbladder and choledochus samples were included as controls. Stomach and duodenum samples were investigated to analyze whether a gastric helicobacter might disseminate to the pancreas in pancreatic cancer patients. Pancreatic specimens were analyzed by Bacteroidesspecific PCR for detecting the translocation of indigenous gut microbes to the diseased pancreas. RESULTS： He/icobacter DNA was detected in pancreas （tumor and/or surrounding tissue） of 75% of patients with exocrine cancer, 57% of patients with neuroendocrine cancer, 38% of patients with multiple endocrine neoplasia, and 60% of patients with chronic pancreatitis. All samples from other benign pancreatic diseases and normal pancreas were negative. Thirtythree percent of the patients were helicobacterpositive in gastroduodenal specimens. Surprisingly, H. bilis was identified in 60% of the positive gastroduodenal samples. All gallbladder and ductus choledochus specimens were negative for helicobacter. Bacteroides PCR-assay was negative for all pancreatic samples. CONCLUSION： Helicobacter DNA commonly detected in pancreatic cancer suggests a possible role of the emerging pathogens in the development of chronic pancreatitis and pancreatic cancer.
瞄准：决定胃、伤寒的 Helicobacter 种类是否与胰腺的癌症被联系。方法：有外分泌的病人胰腺的癌症(n = 40 ) ，神经内分泌癌症(n = 14 ) ，多重内分泌的瘤形成类型 1 (n = 8 ) ，并且慢性胰炎(n = 5 ) 被学习。另外的良性的胰腺的疾病(n = 10 ) 并且正常的胰的标本(n = 7 ) 作为控制被包括。胰腺的织物标本被 Helicobacter 特定的 PCR 试金分析，产品被使中毒描绘坡度电气泳动和定序 DNA。从胰腺的癌症病人的一个子集，象胆囊和管一样的胃或十二指肠的织物我们胆总管织物被分析。胆囊和胆总管样品作为控制被包括。胃和十二指肠样品被调查分析胃的 helicobacter 是否可能在胰腺的癌症病人传播到胰。胰腺的标本被 Bacteroides 特定的 PCR 为检测土生土长的内脏微生物的易位到 diseased 胰分析。结果：Helicobacter DNA 在胰(肿瘤或包围组织) 被检测有外分泌癌症的 75% 病人，有神经内分泌癌症的 57% 病人，有多重内分泌的瘤形成的 38% 病人，和有慢性胰炎的 60% 病人。从另外的良性的胰腺的疾病和正常的胰的所有样品是否定的。百分之 33 个病人在胃与十二指肠的标本是 helicobacter 积极的。令人惊讶地， H。胆汁在 60% 积极胃与十二指肠的样品被识别。所有胆囊和管我们胆总管标本为 helicobacter 是否定的。Bacteroides PCR 试金为所有胰腺的样品是否定的。结论：通常在胰腺的癌症检测的 Helicobacter DNA 在慢性胰炎和胰腺的癌症的发展建议新兴的病原体的一个可能的角色。
基金
Supported by the Swedish Research Council (16×04723), the Royal Physiographic Society in Lund, Lund University Hospital (ALF),and Gunnar Nilssons Cancerstiftelse
