摘要
目的研究青藏高原鼠疫耶尔森菌(鼠疫菌)基因组型分布特征。方法对分离到的青藏高原鼠疫菌297株,根据已经证实的22个差异区段设计引物,每株鼠疫菌的每个基因差异区段都采用PCR技术进行验证。结果在喜马拉雅旱獭鼠疫自然疫源地中,鼠疫菌基因组型有9种,分别为1、5、6、7、8、10、11、新基因组型和Ype-ancestor型,其中以5、8和10型为主,3种基因组型合计所占比例为80．6％(204／253),而且不同地区鼠疫菌基因组型的分布也不一致。青藏高原青海田鼠鼠疫疫源地鼠疫菌基因组型全部为14型。结论青藏高原鼠疫菌基因组型分布具有明显的地理特征。根据基因组型的分布状况推测出了鼠疫菌在青藏高原的传播路径。
Objective To study the distribution of genomovars and microevolutlon of Yersinia pestis in the Qinghai-Tibet Plateau. Methods Primer pairs targeting the twenty-two different regions（DFRs） were designed for detecting the presence or deletion of each DFR in 297 strains isolated from the Qinghal- Tibet Plateau. Results 9 genomovars, i.e. Genomovar 1, 5, 6, 7, 8, 10, 11, new type and Ype ancestor were identified in the Marmota himalayana plague focus of the Qinghai-Tibet Plateau. Among these genomovars,genomovar 5,8 and 10 were dominant types. The total rate of the three genomovars was 80.6% （204/253） and the genomovars in different regions were different. All of 44 strains of Y. pestis in the Microtus fuscus plague focus of the Qinghai-Tibet Plateau belonged to genomovar 14. Conclusion The distribution of genomovars of Y. pestis in the Qinghai-Tibet plateau had remarkable characteristics geographically. Based on the distribution of genomovars of Y. pestis, the routes of transmission and microevolution of Y. pestis were proposed.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2006年第5期412-415,共4页
Chinese Journal of Epidemiology
关键词
鼠疫耶尔森菌
基因分型
差异区段
青藏高原
Yersinia pestis
Genotyping
Different regions
Qinghai-Tibet Plateau
