摘要
AIM： To study the antitumor effect of Chinese compound Jinlongshe （JLS） granules on sarcoma 180 and MKN-45 human gastric cancer cell lines in vivo and its mechanism. METHODS： After establishment of S180 sarcoma （S180） and MKN-45 gastric cancer model of nude mice, the tumor-bearing mice were divided into 5 groups at random. Three experimental groups were respectively given the aqueous extract of JLS granules at doses of 120 g, 60 g and 20 g/（kg per 6/wk,i.g） for 3 wk in S180 and 6 wk in nude mice model. Positive control was given cyclophosphamide （Cy） at a dose of 50 mg/（kg per 3/wk, i.g） for 3 wk in S180 models and 5-Fluorouracil （5-FU） 20 mg/（kg per 3/wk, i.g） for 3 wk in nude mice model. Negative control was given normal saline （NS） at a dose of 0.18 g/（kg per 6/wk, i.g） respectively. After 3 wk in mice bearing S180 tumor and 6 wk in nude mice model, the experimental animals were sacrificed and the masses of tumor were weighed, and the rates of tumor inhibition of each treated group were calculated respectively. To determine the antitumor mechanisms, the morphological changes, cell cycle and apoptosis were observed in MKN-45 nude mice model. Annexin V-FITC/PI double staining FCM assay was used to further determine the live cells, apoptotic cells, necrotic cells and debris. RESULTS： The inhibitory rates of JLS granules at the doses of 20 g/kg, 60 g/kg and 120 g/kg were 50.31%, 55.94% and 68.13% （P 〈 0.01） in nude mice models and 40.90%, 50.32% and 58.46% （P 〈 0.01） in S180 model. The inhibitory rate of Cy was 85.22% in S180 models and the inhibitory rate of 5-FU was 53.43% in nude mice model （P 〈 0.01）. Nuclear chromatin and margination were observed under a transmission electron microscope （TEM）. The G0/G1 phase was arrested, typical apoptotic peak appeared, the apoptotic rate was 22.81%-38.54% in three JLS granule-treated groups. Annexin V-FITC/PI double staining FCM assay showed that the apoptotic cells were 4.36%, 3.08% and 7.08% in three dosages, most cells were localized in the low right quadrant. CONCLUSION：Jinlongshe granules possess anti-tumor effects on experimental tumor models in vivo, and apoptosis induction is one of its anti-tumor mechanisms.
瞄准：到学习，汉语的反肿瘤效果加重 jinlongshe (JLS ) 肉瘤 180 上的小粒和 MKN-45 人的胃的癌症房间线在活体内和它的机制。方法：在 S180 肉瘤(S180 ) 和胃的癌症裸体老鼠建模的 MKN-45 的建立以后，忍受肿瘤的老鼠在随机被划分成 5 个组。三个试验性的组分别地在 120 g， 60 g 和 20 g/ 的剂量被给 JLS 小粒的水浸膏(kg 每 6/wk， i.g ) 为在 S180 的 3 wk 和在裸体的 6 wk，老鼠当模特儿。积极控制在 50 mg/ 的剂量被给出租机动三轮车磷酰胺(Cy )(kg 每 3/wk， i.g ) 为在 S180 模特儿和 5 氟尿嘧啶(5-FU ) 的 3 wk 20 mg/(kg 每 3/wk， i.g ) 为在裸体的 3 wk，鼠标当模特儿。否定控制在 0.18 g/ 的剂量被给生理盐水(NS )(kg 每 6/wk， i.g ) 分别地。在在忍受 S180 肿瘤的老鼠的 3 wk 和在老鼠建模的裸体的 6 wk 以后，实验动物被牺牲，肿瘤的群众被称，并且每个对待的组的肿瘤抑制的率分别地是计算的。为了决定反肿瘤，机制，词法变化，房间周期和 apoptosis 在老鼠建模的 MKN-45 裸体被观察。Annexin V-FITC/PI 两倍染色的 FCM 试金被用来进一步决定实时房间， apoptotic 房间，坏死的房间和碎片。结果：在 20 g/kg， 60 g/kg 和 120 g/kg 的剂量的 JLS 小粒的禁止的率是 50.31% ， 55.94% 和 68.13%(P < 0.01 ) 在裸体老鼠模特儿并且 40.90% ， 50.32% 和 58.46%(P < 0.01 ) 在 S180 模特儿。Cy 的禁止的率在 S180 模特儿是 85.22% ， 5-FU 的禁止的率在老鼠建模的裸体是 53.43%(P < 0.01 ) 。原子染色质和着边在一台传播电子显微镜(TEM ) 下面被观察。G0/G1 阶段被逮捕的、典型 apoptotic 山峰出现了， apoptotic 率是在三个 JLS 对待小粒的组的 22.81%-38.54% 。两倍染色的 FCM 试金显示出的 Annexin V-FITC/PI apoptotic 房间在三剂量是 4.36% ， 3.08% 和 7.08% ，大多数房间在低正确象限是局部性的。结论：Jinlongshe 小粒在试验性的肿瘤模特儿在活体内，和 apoptosis 正式就职上拥有反肿瘤效果是它的反肿瘤机制之一。
基金
Supported by the Modernization Programs of Chinese Materia Medica of Science and Technology Commission Foundation of Shanghai, No. 04DZ19811
