摘要
目的探讨被动吸烟对胚胎的致畸作用,并建立一个理想的被动吸烟致畸的动物模型方法采用自制的半封闭式被动吸烟染毒箱,对不同孕期的金黄地鼠以不同时间及不同剂量的分组组合,给予烟熏处理后,进行致畸效果的观察分别计数各组孕鼠的胚胎植入数活胎、吸收胎、死胎及致畸率,并进行统计学分析。结果被动吸烟对胚胎有明显的致畸作用(χ2=86.23,P<0.05或P<0.01),孕6、7、8d期间,每日3次,共6h的烟熏,其致畸率最高,为41.6%,吸收胎数相对较低,活胎平均体重最低。结论孕6、7、8d的金黄地鼠,每日3次,共6h的烟熏,致畸率高,吸收率较低,可产生严重的畸形,是一个比较理想的被动吸烟致畸动物模型。
Objective ： To study the teratogenic effects of passive smoking on embryo and to establish a good animal model of passive smoking - induced malformation. Methods ： In the chamber made by ourself for passive smoking experiment, gold hamsters of different gestational period were exposed to tobacco smoke in different time period and different doses, then the number of implantations, livings, absorbed fetuses, deaths and the rate of malformation were counted for each group and dealt with statistical analysis. Results ： Passive smoking could induce teratogenesis （χ^2 = 86. 23, P 〈0. 05 or P 〈 0. 01 ）, the gold hamster were exposed to tobacco smoke from embryo day 6 to 8.3 times a day, 6 hours in total. The malformation rate as 41.6% which was significantly higher than other group. The average body weight of living fetuses was lowest and the number of absorbed fetuses were lower than other group. Conclu- sion： The gold hamsters in embryonic day 6 to 8 exposed to tobacco smoke 3 time a day, 6 hours in total can cause higher teratogenic rate, relatively lower resorption rate and severe malformations. So it is a good animal model for passive smoking induced malformation.
出处
《中国优生与遗传杂志》
2006年第6期89-90,共2页
Chinese Journal of Birth Health & Heredity
关键词
被动吸烟
金黄地鼠
胚胎
致畸
动物模型
Passive smoking
Gold hamsters
Embryo
Teratogenesis
Animal model
