摘要
目的研究骨膜、滑膜和软骨组织对软骨细胞蛋白多糖、型胶原和核因子κB(nuclearfactorkappa B,NF-κB)等基因表达的影响,探讨各种组织对软骨再生的作用。方法20只新西兰兔,于膝关节切取20个4mm×4mm×4mm软骨块、25个2mm×2mm×2mm小软骨块、25个2mm×2mm滑膜片和25个2mm×2mm骨膜块。将4mm×4mm×4mm软骨块分别置于培养皿孔中央进行培养,按软骨块周围放置的组织块不同分为A、B、C、D4组(n=5)。A组,周围放置5个2mm×2mm的滑膜片;B组,周围放置5个2mm×2mm的骨膜片;C组,周围放置5个2mm×2mm×2mm的小软骨块;D组,周围未放置任何组织块,作为对照组。培养7d后,提取中央软骨块细胞RNA,采用RT-PCR技术,检测4组软骨细胞蛋白多糖、型胶原和NF-κBmRNA转录情况。结果A组蛋白多糖mRNA转录减少,基因表达相对密度值为1.09±0.21,与D组1.25±0.25比较差异有统计学意义(P<0.05),型胶原及NF-κBmRNA转录亦减少,基因表达相对密度值与D组比较差异无统计学意义(P>0.05);B组蛋白多糖、型胶原及NF-κBmRNA转录增高,基因表达相对密度值分别为1.60±0.26、1.57±0.24及4.20±2.22,与D组比较差异有统计学意义(P<0.05);C组型胶原基因表达相对密度值为1.43±0.28,与D组比较差异有统计学意义(P<0.05),蛋白多糖及NF-κB基因表达相对密度值与D组比较差异无统计学意义(P>0.05)。结论骨膜具有刺激软骨细胞蛋白多糖、型胶原和NF-κBmRNA转录的能力。NF-κB及其信号传递系统很可能参与了骨膜对软骨再生的促进过程。
Objective To study the effects of the periosteum, synovium and cartilage tissues on the gene expressions of proteoglycan, collagen Ⅱ , and nuclear factor kappa B （NF- κB） and to investigate the different effects of these tissues on cartilage regeneration. Methods In 20 New Zealand white rabbits, 20 cartilage explants were taken from the knee joints in each rabbit, the size of which was 4 mm×4 mm×4 mm. All the cartilages were divided into the following 4 groups and cultured for 7 days： Group A, with 5 pieces （2 mm× 2 mm） of the synovium of the knee joints in each dish; Group B, with 5 pieces （2 mm×2 mm） of the periosteum in each dish; Group C, with 5 pieces （2 mm× 2 mm×2 mm） of the cartilage in each dish; and Group D, with no addition of other tissues （control group）. RNA was extracted from the cells of the cartilage explants （4 mm × 4 mm × 4 mm） in all the dishes. The gene expressions of proteoglycan, collagen Ⅱ and NF -κB were defected by a reverse transcription-polymerase chain reaction （RT-PCR）. Results In group A, the gene expression of proteoglycan was significantly decreased. The relative density of this gene expression had a significant difference when compared with that in group D （1.09±0.21 vs. 1.25±0. 25, P〈0. 05） ; the gene expressions of collagen Ⅱ and NF-κB were also decreased, but they had no significant differences when compared with those in group D （P〉0. 05）. In group B, the gene expressions of proteoglycan, collagen Ⅱ , and NF-κB were significantly increased. The relative densities of these gene expressions were 1.60±0.26, 1.57± 0.24, and 4. 20± 2.22, respectively, which had significant differences when compared with those in group D （P〈0.05）. In group C, the relative density of the gene expression of collagen Ⅱ was 1.43±0. 28, which had a significant difference when compared with that in group D （P〈0.05）, but the relative densities of the gene expressions of proteoglycan and NF-κB had no significant differences when compared with those in group D （P〉0. 05）. Conclusion The results indicate that the periosteum can up-regulate the gene expressions of proteoglycan, collagen Ⅱ and NF-κB. The NF-κB is likely to be an important nuclear transcription factor related to cartilage regeneration. The results also suggest that the periosteum may be better in facilitating the cartilage repair and regeneration in clinical practice.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第6期670-674,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
南京市科技局国际合作处资助项目(200301109)~~
关键词
骨膜
滑膜
软骨细胞
再生
基因表达
Periosteum Synovium Chondrocytes Regeneration Gene expression
