摘要
本文用离子对ＨＰＬＣ法测定了天仙子中莨菪碱和东莨菪碱的含量。莨菪碱和东莨菪碱的样品量与峰面积积分值的线性范围分别为０．２４４５～０．７３３２μｇ，ｒ＝０．９９９５和０．１８６４～０．５５９２μｇ，ｒ＝０．９９９９；平均回收率分别为：９８．８２％，ＲＳＤ＝３．４１％（ｎ＝５）和９７．８６％，ＲＳＤ＝１．８５％（ｎ＝５）。样品被处理后，所得色谱图分离度好，无干扰。并用ＴＬＣ法对天仙子（含中亚天仙子）和南天仙子进行了鉴别。为评价天仙子的真伪优劣提供了科学依据。
The contents of hyoscyamine and scopolamine in the seeds of Hyoscyamus niger were determined by applying reverse phase ion-pair HPLC. In the scope of 0. 2445～0. 7332μg. and 0. 1864～0. 5592μg, the contents of hyoscyamine and scopolamine had good linear relationship with their peak areas. r=0. 9995 and r=0. 9999. The average recoveries were 98. 82%, RSD = 3. 41%, n= 5 and 97. 86%, RSD= 1. 85%, n= 5 respectively. After the sample was purified.the resolution of the chromatogram was very good and there was no any interference in it. in addition. Hyoscyamus niger, H. pusillus and Hygrophila megalantha were identified by TLC.
出处
《药物分析杂志》
CSCD
北大核心
1996年第2期119-122,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱
莨菪碱
东莨菪碱
天仙子
南天仙子
reverse phase ion-pair HPLC
hyoscyamine
scopolamine
Hyoscyamus niger
Hygrophila megalantha
