摘要
目的探讨肿瘤坏死因子α(TNFα)及β(TNFβ)基因多态性与多发性硬化(MS)的相关性。方法采用序列特异性引物和聚合酶链式反应(PCR)、限制性内切酶酶切技术对60例MS患者(MS组)和110例健康者(对照组)的TNFα及β基因多态性进行分析。结果MS组TNFα-308等位基因分布频率与对照组比较有显著性差异(P=0．04),且MS组等位基因A含量(3．3%)与对照组(11．8%)比较也有显著性差异(P=0．008)；MS组TNFβ+252等位基因分布频率与对照组比较有显著性差异(P=0．014),且MS组等位基因A含量(69．2%)与对照组(55．5%)比较也有显著性差异(P=0．014)。结论TNFα-308 A等位基因的多态性与MS的发病有一定负相关,TNFβ+252 A等位基因频率及TNFβ+252 A/A基因分布频率与MS的发病有一定正相关。
Objective To investigate the correlation between tumor necrosis factor α （TNFα） and β （TNFβ） gene polymorphisms and multiple sclerosis （MS）. Methods TNFα and TNFβ gene polymorphisms were analyzed in 60 MS patients （MS group） and 110 healthy people （control group） with sequence specific primer and polymerase chain reaction （PCR）, and restriction endonuclease technology. Results There was a significant difference in TNFα-308 allelic gene frequency between MS group and control group（P=0.04）, and TNFα allelic gene A content in MS group（3.3%） was also significantly different from that in control group （11.8%, P=-0.008）. There was a significant difference in TNFβ＋252 allelic gene frequency between the 2 groups（P=0.014）, and TNFβ allelic gene A content in MS group（69.2%） was also significantly different as compared with control group （55.5%, P=0.014）. Conclusion There was a negative correlation between MS group and control group in TNFα-308 A allelic gene frequency, but a positive correlation in TNF-β＋252 A allelic gene frequency and TNF-β＋252 A/A gene frequency.
出处
《中华神经医学杂志》
CAS
CSCD
2006年第6期572-575,共4页
Chinese Journal of Neuromedicine
