摘要
用高效液相色谱法,建立喘平滴丸中主要成分的含量测定方法.色谱柱Eclipse XDB-C8（4.6×150 mm,5 μm）,流动相为甲醇-0.02 mol/L磷酸二氢钾溶液-0.04 mol/L磷酸溶液（体积比为1/9.5/9.5）,流速为1.0 mL/min,比较了检测波长207,210,216 nm的HPLC图谱,遴选最佳检测波长.在检测波长207 nm下,对照品盐酸麻黄碱、盐酸伪麻黄碱、氢溴酸东莨菪碱分别在11～110、10～100、2.5～25 ng范围内与色谱峰面积呈良好的线性关系：r=0.999 8.平均加样回收率分别为99.55%、99.05%、98.53%,RSD分别为1.56%、1.79%、1.43%（n=5）.本法简便、快速,适用于喘平滴丸的质量控制和含量测定.
To determine the main components in Chuanping dripping pills. HPLC was applied and chromatographic conditions were established as follows： chromatographic column Eclipse XDB -C8 （4. 6 × 150 mm, 5 μm）, mobile phase MeOH -0.02 mol/L KH2PO4 -0. 04 mol/L H3PO4 （1/9.5/9.5）, flow rate 1.0 mL/min, and detection wavelength 207 nm. The standards of ephedrine hydrochloride, pseudoephedrine hydrochloride and hyoscine hydrobromide showed good linear response with r =0. 999 8 in the range of 11 - 110 ng, 10 - 100 ng and 2. 5 -25 ng, respectively. The average recovery ratio were 99. 55%, 99.05%, 98.53%, and RSD （n =5） were 1.56%, 1.79% and 1.43% for samples of ephedrine hydrochloride, pseudoephedrine hydrochloride and hyoscine hydrobromide respectively. The method can be used for the control and determination of Chuanping dripping pills.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期62-64,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(30472198)
国家中医药管理局基金资助项目(04-05ZP50)
广东省自然科学基金资助项目(04105450)
关键词
喘平滴丸
主要成分
含量测定
chuanping dripping pills
main component
content determination
