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酵母双杂交系统的发展及其衍生系统的比较 被引量：4

The Development of Yeast Two-Hybrid System and it's Several Derived Systems
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摘要 酵母双杂交及其衍生系统是鉴定及分析蛋白质-蛋白质、蛋白质-DNA、蛋白质-RNA相互作用的最常用、最有效的工具之一。本文系统介绍了该技术的背景、发展过程,以及由Fields和Song初次描述的由酵母双杂交系统衍生而来的几种主要的双杂交系统的特点,并简要比较了各系统的优缺点。 Yeast two-hybrid system and other systems derived from it have become one of the most popular and powerful tools to study biological macromolecular interactions. This backgrounds, development and characteristic of this technique were reviewed in this paper. In addition, several useful systems derived from the yeast two-hybrid system which was firstly described by Fields and Song in 1989 would be compared here.
作者 高宁 胡宝成
机构地区 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2006年第3期421-424,共4页 Letters in Biotechnology
基金 国家自然科学基金项目(30370441)
关键词 酵母双杂交 蛋白质相互作用 yeast two-hybrid protein-protein interactions
分类号 Q789 [生物学—分子生物学]
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参考文献24

  • 1Young KH.Yeast two-hybrid:so many interactions,(in) so little time[J].Biol Reprod,1998,58(2):302
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同被引文献54

  • 1张爱香,季静,王罡,张艳贞.枸杞叶片cDNA文库的构建[J].吉林农业大学学报,2005,27(2):155-157. 被引量：10
  • 2胡标林,李名迪,万勇,朱雪晶,张铮.我国水稻抗旱性鉴定方法与指标研究进展[J].江西农业学报,2005,17(2):56-60. 被引量：55
  • 3秘彩莉,张学勇,温小杰,刘旭.利用cDNA-AFLP技术获得小麦耐盐性相关基因TaVHA-C[J].中国农业科学,2006,39(9):1736-1742. 被引量：21
  • 4Fields S,Song O.A novel genetic system to detect protein-protein interctions.Nature,1989,340(6230):245-246.
  • 5Brachmann R K,Boeke J D.Tag games in yeast:the two-hybrid system and beyond.Current Opinion in Biotechnology,1997,8(5):561-568.
  • 6Rain J C,Crabier A,Gérard A,et al.Yeast two-hybrid detection of integrase-host factor interactions.Methods,2009,47(4):291-297.
  • 7Pajunen M,Poussu E,Turakainen H,et al.Application of Mu in vitro transposition for high-precision mapping of protein-protein interfaces on a yeast two-hybrid platform.Metheds,2009,49(3):255-262.
  • 8Keegan L,Gill G,Ptashne M.Separation of DNA bind from the transcription-activating function of eukaryotic regulatory protein.Science,1986,231(4739):699-704.
  • 9Ma J,Ptashne M.A new class of yeast transcriptional activators.Cell,1987,51(1):113-119.
  • 10Fields S,Sternglanz R.The two-hybrid system:An assay for protein-protein interactions.Trends Genet,1994,10(8):286-292.

引证文献4

二级引证文献9

生物技术通讯
2006年 第3期

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