摘要
利用Boyden小室法和FACS分析法,我们分析了五株小鼠胸腺基质细胞系(MTSC)培养上清液对中性粒细胞,单核巨噬细胞和淋巴细胞的化学趋化因子(Chemokines)活性,及定向迁移的淋巴细胞中B细胞、CD 4^+CD 8^-和CD4^-CD8^+T细胞的比例。结果显示,五株MTSC的培养上清液对上述靶细胞均有不同程度的趋化作用.MTSC细胞分泌趋化因子的情况可分为三类:1.MTEC 1和MTEC 2产生的Chemokine(s)对中性粒细胞和淋巴细胞的趋化作用相对较强;2.MTDC 4分泌的Chemokine(s)主要作用于单核巨噬细胞;3.MTEC 3和MTEC 5分泌的Chemokine(s)对多种类型的靶细胞,包括中性粒细胞、单核巨噬细胞和淋巴细胞,表现的趋化作用没有明显的强弱之分。MTSC-SN对B细胞的趋化活性普遍高于对T细胞的趋化活性,对CD 4^-CK 8^+T细胞的趋化活性高于对CD 4^+CD 8^-T细胞的趋化活性。MTSC-SN中趋化因子的分析,有利于新型chemokines的发现及其生物功能的阐明,并可进一步研究Chemok-ine(s)在T细胞发育中的作用。
By using Boyden Chamber and PACS methods, we analysed the chemotactic activity of five mouse thymic stromal cell (MTSC) lines' culture supernatant for nautrophil, monocyte/macrophage and lymphocyte, and the phenotype of the attracted lymphocytes. MTEC1, MTEC 2. MTEC 3, MTEC 5 are mouse thymus epithelial cell lines. MTDC 4 is a mouse thymus dendritic cell line. The results indicate that all of the five MTSC super-natants have chemotactic activity for the target cells mentioned above but in different degree. Chemokines produced by the MTSC cells can be classified into three categories: (1) Chemokines produced by MTEC 1 and MTEC 2 are more potent
for the attraction of neutrophils and lymphocytes. (2) MTDC 4 derived chemokines are highly potent for the attraction of monpcytes/macrophoges. (3) Chemokines produced by MTEC 3 and MTEC 5 are equally attractive to neutrophils, lymphocytes and monocytes/macrophages. Most of the MTSC-SN are more potent for the migration of B lymphocyte than for T lymphocyte. The MTSC-SN exhibits higher chemotactic activity to CD 4~CD 8+ T cell subset than to CD4+CD8- T cell subset. The characterization of chemokines in MTSC-SN will benefit the finding of new chemokines and the analysis of the mechanism of thymus homing.
出处
《实验生物学报》
CSCD
1996年第1期25-32,共8页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
洛氏基金(90-523)
关键词
鼠
小鼠
趋化因子
胸腺基质细胞
chemokines. mouse thymic stromal cell line. lymphocyte, monocyte/macro-phage. neutrophil
