摘要
目的系统观察过敏性紫癜(HSP)急性期调节性T细胞(Tr)亚群及辅助性T细胞亚群(Th1/Th2)的变化,探讨HSP急性期免疫失衡的发病机制。方法流式细胞术检测20例HSP急性期患儿各种调节性T细胞亚群(CD4+CD25+Tr、Tr1、Th3等)和辅助性T细胞亚群(Th1、Th2)的改变,并采用逆转录聚合酶链反应(RT-PCR)和荧光定量聚合酶链反应(Real-tim e PCR)检测其外周血单个核细胞(PBMC)Foxp3 mRNA的表达。同期20例同龄健康儿童作为对照。结果HSP急性期CD3+CD8-INF-γ-IL-4+(Th2)细胞显著增高(P<0.05),Th1/Th2比值显著降低(P<0.05)。各调节性T细胞亚群CD4+CD2+5Tr、CD4+IL-4-IL-10+(Tr1)、CD4+TGF-β+(Th3)细胞与正常对照组比较均显著降低(P均<0.05)。HSP组PBMC Foxp3 mRNA的表达与正常对照组比较亦显著降低(0.22±0.05vs.66.32±9.25,P<0.001)。结论HSP急性期存在明显的免疫失衡,Th2优势明显;调节性T细胞CD4+CD2+5Tr、Tr1、Th3数量减少导致的免疫抑制效应不足可能是导致HSP免疫失衡的重要原因,而HSP患儿调节性T细胞减少与Foxp3表达降低有关。
Objective Henoch-Schonlein purpura （HSP） is one of the most common small vessel forms of autoimmune vasculitis in children. Immunologic derangement including humoral and cellular immunity disequilibrium and proinflammatory factors dysfunction are involved in the acute stage of HSP. Recently data revealed that regulatory T cells （Tr） play a pivotal role in preventing development of autoimmune and allergic diseases. This study aimed to explore the role of Tr cells in pathogenesis of HSP and the factors affecting development of Tr cells. Methods Twenty patients with HSP and 20 age-matched healthy children were enrolled into this study. Flow cytometric （FCM） analysis was performed to detect the pementage of regulatory T cells subpopulation （including CD4^＋CD25^＋Tr, Trl and Th3 ） and helper T cells subpopulation （Thl and Th2 ）. Reverse transcription-polymerase chain reaction （RT-PCR） and real-time PCR were used to analyze Foxp3 expression in peripheral blood mononuclear cell （PBMC）. Results Compared with healthy control subjects, the proportions of Th2 cell in patients with HSP were significantly higher （ P 〈 0. 05）, and the ratio of Th1/Th2 was remarkably decreased （P 〈 0. 05 ） . The proportions of three subpopulation of regulatory T cells including CD4^＋CD25^＋ Tr, Tr1, Th3 in patients with HSP were all significantly lower than those of controls （ P 〈 0. 05 ）. The mRNA expression of Foxp3 in patients with HSP showed similar tendency （ P 〈 0. 001 ）. Conclusions The decrease of three subpopulations of regulatory T cells might be involved in pathogenesis of HSP and associated with the decreased expression of Foxp3 gene.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2006年第6期411-414,共4页
Chinese Journal of Pediatrics
