摘要
研究苏丹红Ⅰ、Ⅲ和Ⅳ对人肝癌细胞HepG-2,胃癌细胞SGC-7901增殖的影响.利用激光共聚焦显微镜及三维图像分析HepG-2细胞受到苏丹红Ⅰ、Ⅲ和Ⅳ不同剂量作用后DNA和RNA含量的比值,流式细胞仪测定SGC-7901细胞DNA含量的变化,分析细胞周期的变化.结果表明,对照组细胞DNA/RNA比值为1.223 2±0.084 4,苏丹红Ⅰ、Ⅲ和Ⅳ处理后的HepG-2细胞DNA荧光染色强度明显高于RNA,以低剂量组的作用显著(p<0.01),其DNA/RNA比值分别为1.609 6±0.199 0,1.445 5±0.163 3,1.708 1±0.109 0.三维图表明,HepG-2细胞DNA荧光多聚集于细胞核区域,较RNA浓集、荧光强度大;可促进SGC-7901细胞由G1期进入S期和G2期,表现为G1期细胞平均DNA含量下降,S期DNA含量上升,G2期DNA含量上升,细胞增殖指数(PI)增加,S期细胞(SPF)指数增加.苏丹红Ⅰ、Ⅲ、Ⅳ可以通过影响细胞周期,从而调节细胞的增殖活动,促进细胞分化增殖.
To study the effect of sudan Ⅰ ,Ⅲ and Ⅳ on the growth of HepG-2 and SGC-7901, the ratio of DNA/RNA and 3D image in HepG-2 treated by different dose of sudan Ⅰ ,Ⅲ and Ⅳ was detected by LCM, the content change of DNA and the cell cycle in SGC-7901 treated by different dose of sudan Ⅰ ,Ⅲ and Ⅳ was detected by flow cytometry. Results showed the ratio of DNA/RNA in control group was 1.223 2 ± 0.084 4,after treated by sudan Ⅰ ,Ⅲ and Ⅳ the fluorescence intensity of DNA was stronger than RNA, and the low dose group was very marked compared with control group（ p 〈 0.01 ）, the ratio was 1.609 6 ±0. 199 0, 1.445 5 ±0.163 3 ,and 1. 708 1 ± 0. 109 0. 3D image showed that the DNA fluorescence mostly assembles on nucleolus, which was denser and stronger than RNA. Sudan Ⅰ ,Ⅲ and Ⅳ accelerated the cell cycle from G1 phase to S and （32 phase, the average content of DNA in G1 phase decreased and in S and （32 phase increased, the index of PI and SPF increased. Sudan Ⅰ ,Ⅲ and Ⅳ could affect cell cycle and advanced its differentiation and proliferation.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2006年第6期1201-1207,共7页
Environmental Science
基金
国家自然科学基金项目(30400592
30400352)
哈尔滨市科技局后备带头人基金项目(2004AFXXJ030
2004AFQXJ035)
