摘要
建立了特异性强的肺炎克雷伯氏菌荚膜多糖全菌ELISA检测方法,检测结果与多糖表达量相关性好;以全菌ELISA值结合菌数为评价指标,对影响荚膜多糖表达的培养基组成及发酵条件进行了优化,优化后的摇瓶培养条件下发酵液活性和生物量分别比优化前提高72.7和33倍,并经7L罐放大实验,绘制发酵动力学曲线,为肺炎克雷伯氏菌荚膜多糖进一步开发打下基础。
In order to evaluate the fermentation process of Klebsiella pneumoniae, indirect cell ELISA was established and investigated. It showed a good correlation to the yield of active capsular polysaccharide. Both indirect cell ELISA and biomass were utilized to evaluate the fermentation process, The influence of substrate composition and growth conditions on CPS expression by KlebsieUa pneumoniae using submerged cultivation were investigated systematically, The statistical optimization of media and growth conditions in shake flask were established. The biomass and activity of the culture under the optimum process were enhanced by 72.7 and 33 times respectively. Then the process was magnified and improved in stired tank fermenter.
出处
《工业微生物》
CAS
CSCD
北大核心
2006年第2期16-21,共6页
Industrial Microbiology
