摘要
为控制黄曲霉毒素主要产生菌寄生曲霉AS3.4407在粮食储藏环境中的产毒污染,采用组合实验设计策略,考察了五个模拟环境因子对菌株产毒的影响。首先,利用Plack-ett-Burman试验筛选出三个对毒素产量影响显著的因子(转速、温度、pH),在此基础上,采用CCD实验设计法(Central Composite Design,CCD)研究了这三个因子对寄生曲霉AS3.4407产毒影响的交互作用,并根据毒素曲面方程和二次多项回归方程得到该菌的最低产毒条件。在环境通风良好(转速240rpm),储藏温度较低(18℃),pH为弱碱性(pH 8)条件下,寄生曲霉AS3.4407产毒量最低,验证试验证实了该方程的预测值与实际值之间具有较好的拟合度。为粮库采取相应措施,改变储藏环境条件以降低毒素污染提供了很好依据。
In order to control the aflatoxin contamination produced by Aspergillus parasiticus AS3. 4407 during grain storage, the effect of five environmental factors on the production of aflatoxin were studied by the composite experimental design. Three key factors （rotatory speed, temperature, pH） were screened out by the Plackett- Burman method and at the basis of which, the interaction effect of these factors on the production of aflatoxin were studied by CCD method. The environmental condition of the least aflatoxin production were acquired from surface equation and regression equation, that is rotatory speed 240rpm, 18℃ and pH 8. Results of validate experiment proved that there are good fitness between the predicted and actual value. Useful reference were provided by these results for the control of aflatoxin contamination.
出处
《粮食储藏》
2006年第3期39-42,共4页
Grain Storage
