摘要
以6个银杏种质为试材,通过对影响AFLP技术的多种因素进行研究,建立了一套适合银杏的AFLP技术优化体系。优化的关键因素为:①模板DNA的质量:经多次抽提纯化,将DNA沉淀挑出,而不采用离心方法,获得高纯度的DNA;②酶切时DNA模板为300 ng,反应时间为6 h;③连接最适反应时间为10 h;④预扩模板即连接产物最佳用量为1μl;⑤预扩增产物最适稀释倍数为70倍。
Some factors affecting the AFLP analysis system for six Ginkgo bilola germplasms were studied, and an optimized system was established. The important factors in the system are ： ①quality of template DNA. ②300 ng of template DNA in the digest system and 6 hours for the reaction. ③at least 10 hours for ligation. ④one microlitre ligation products as the template of preamplification. ⑤70 times of dilution for the products of preamplification with 1 XTE for selective amplification.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2006年第3期377-381,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
林业部948项目(98-4-16)
关键词
银杏
AFLP
体系优化
Ginkgo bilola
AFLP
optimization of the system
