摘要
为了观察β-1，4-半乳糖基转移酶-Ⅰ（β-1，4-galactosyltransferase-Ⅰ，β-1，4-GalT-Ⅰ）在正常和钳夹伤后大鼠坐骨神经中表达的变化，本研究采用RT—PCR方法，从小鼠坐骨神经中特异性地扩增β-1，4-GalT-ⅠcDNA片段并将其克隆到pGEM-T载体。采用体外转录的方法合成地高辛标记的正、反义β-1，4-GalT-ⅠRNA探针。通过原位杂交和图像分析的方法，观察β-1，4-GalT-ⅠmRNA在正常和夹伤大鼠坐骨神经中的表达及其变化。结果表明：β-1，4-GalT-Ⅰ mRNA在大鼠坐骨神经的髓鞘中表达，在夹伤坐骨神经后1-2d，β-1，4-GalTⅠmRNA在坐骨神经的表达最高，于夹伤后1周开始下降，在夹伤后1个月恢复至正常水平。上述结果提示坐骨神经夹伤后β-1，4-GalTⅠmRNA的表达发生变化，并且主要表达在坐骨神经的Schwann细胞。本研究结果为进一步分析β-1，4-GalT-Ⅰ在周围神经再生中的调控机制奠定了基础。
To study the potential role of β-1,4-galactosyltransferase-Ⅰ （ β-1,4-GalT-1） in nerve regeneration and resumption, we preliminarily investigated the expressive changes of β-1,4-GalT-Ⅰ mRNA in normal and crashed sciatic nerves in the rat. We amplified the cDNA fragments of β-1,4-GalT-Ⅰ in mouse sciatic nerves by RT-PCR. The products of RT-PCR were subcloned into pGEM-T vector, confirmed by sequencing, which was used to label probes for in situ hybridization. The data indicated that β-1,4-GalT-Ⅰ mRNA was localized mainly in the sheath of the sciatic nerve by in situ hybridization. It was also found that the expressive changes of β-1,4-GalT-Ⅰ mRNA in crushed sciatic nerves reached the peak at 1-2 days after the nerve injury and began to decrease at 1 week thereafter and returned to normal level at 1 month after the nerves injury by image analysis. These results suggest that β-1,4-GalT-Ⅰ may localize mainly in Schwamn cells of the sciatic nerves. It may play a role in the peripheral nerve regeneration at the early stage after the sciatic nerve injury in the rot.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第3期291-295,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30300099)
江苏省自然科学基金(BK2003035)
江苏省教育厅自然科学项目(03KJB180109)
南京医科大学校科技发展基金(Ny04002)资助项目
