摘要
目的建立健脾补血片中阿魏酸的高效液相色谱(HPLC)测定方法。方法采用HPLC法。色谱柱Lichrospher C18(4.6 mm×250 mm,5μm),C18保护柱;流动相:乙腈-0.378 mol/L冰醋酸水溶液(30∶70);检测波长321 nm;流速0.8 mL/min;柱温:室温;进样量10μL。结果阿魏酸质量浓度在1.0-10.0 mg/L范围内,与峰面积呈良好的线性关系(r=0.9996),平均回收率为99.8%,RSD为0.26%。结论HPLC可用于健脾补血片中阿魏酸的质量浓度测定。
Objective To establish a determination method of ferulic acid in Jianpibuxue tablets. Methods HPLC was used with Lichrospher C18 （4.6 mm× 250 mm, 5 μm） as the column. The mobile phase was a mixture of acetonitrile-0. 378 mol/L acetum （30 ： 70） and in a flow rate of 0.8 mL/min; The detective wavelength was 321 nm Column temperature was room temperature and the injection quantity was 10 μL. Results Ferulic acid in samples was well separated. The linear range was 1.0-10.0 mg/L（r=0. 9996）. The average recovery was 99.8% and RSD was 0.26 %（n=6）. Conclusion This method can be used for quality control of ferulic acid in Jianpibuxue tablets.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期304-306,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
