摘要
目的探讨参附注射液对大鼠缺血再灌注心肌组织内核转录因子-кB(NF-кB)活性、细胞间粘附分子-1(ICAM-1)和白细胞介素-6(IL-6)水平的影响。方法健康SD大鼠36只随机分为三组,空白对照组(S组)、缺血再灌注组(I/R组)、参附注射液组(SF组),每组12只。采用钳闭左冠状动脉前降支制备心肌缺血再灌注模型。电镜观察超微改变,免疫组织化学法检测心肌NF-кB活性和ICAM-1水平,ELISA法检测大鼠心肌IL-6水平。结果1.电镜观察超微改变:I/R组部分肌纤维断裂,肌节模糊,线粒体肿胀,部分线粒体膜破裂,核膜完整高度皱缩,染色质浓缩边聚、呈块状;但SF组上述病变程度明显减轻。2.S组和SF组NF-кB活性、ICAM-1和IL-6水平显著低于I/R组(P<0.01),但SF组NF-кB活性和IL-6水平高于S组(P<0.05)。结论参附注射液能抑制缺血再灌注心肌NF-кB活性和降低ICAM-1和IL-6水平,减轻缺血再灌注心肌炎症反应。
Objective To study the effect of ShenFu injection on the activty of NF- κB and the level of ICAM - 1 and IL -6 following acute myocardial ischemic reperfusion. Methods 36 SD rats were randomized into three groups each consisting of ：12 rats： sham group（S group）, ischemic reperfusion group（ I/R group）, ShenFu injection （SF group）,. Ischemic Reperfusion models were made by ligated left coronary artery. Immunohistochemistry detected the activty of NF-κB and the level of ICAM -1. ELISA method measured IL-6 level. Results Electron microscope change：There were mitochondria swel group ,but less change in SF group. The activity of and SF group were significantly lower than those , rupture of mitochondrial membrane in I/R NF-κB ,the level of ICAM -1 and IL-6 in S n I/R group（P 〈0. 01 ）,and the activity of NF -KB, the level of IL -6 of myocardial tissue in SF group were higher than in S group （P 〈 0. 05 ）. Conclusion ShenFu injection can inhibit the activity of NF - κB, decrease ICAM - 1 and IL -6 level, protect myocardial injury after ischemic reperfusion.
出处
《湖北民族学院学报（医学版）》
2006年第2期10-11,14,共3页
Journal of Hubei Minzu University(Medical Edition)
关键词
参附注射液
心肌
缺血再灌注
细胞因子
ShenFu injection
Myocardial ischemic reperfusion
cytokine
