摘要
目的探讨骨髓干细胞动员剂粒细胞集落刺激因子(granulocyte-colony-stimulating factor,G-CSF)对实验性心肌梗死(心梗)大白兔心脏结构和功能的保护作用。方法大白兔40只,结扎冠状动脉前降支,于心电图上出现R波逐渐降低,ST段弓背向上抬高和病理性Q波时,证明心梗模型成功。将大白兔随机分成两组,每组20只。实验组于心梗模型建立后1 h给予G-CSF皮下注射,连续7 d,对照组建立心梗模型后1h仅注射等量生理盐水。术后24 h及4周心脏超声观察心功变化。处死动物,取出心脏行组织病理学分析,测定两组梗死面积的大小、毛细血管密度以及观察心肌纤维化变化。结果实验组在术后4周时射血分数(EF)、左室短轴缩短率(FS)、左室运动幅度(LVWE)、收缩末左室后壁增厚率(LVWTs)等心功能指标均较对照组有明显的改善,其中射血分数增加13％(实验组为63．40％,对照组仅为50．51％)。HE染色显示,对照组心肌纤维排列紊乱且不规则;实验组心肌纤维排列有序,梗死灶面积较对照组减小了30.2％,毛细血管密度增加了30．7％,Masson染色蓝色的胶原纤维明显少于对照组。结论急性心梗时给予G-CSF,可有效增加梗死区及周围毛细血管密度,缩小梗死面积,减少胶原纤维形成,稳定心脏结构及改善心功能。骨髓干细胞动员剂为急性心肌梗死的内科治疗开辟了一条新途径。
Objective To explore the effect of granulocyte colony-stimulating factor （G-CSF） on cardiac structure and heart function in rabbits with acute myocardium infarction. Methods The 40 white Japanese big-ear rabbits were divided two groups,with 20 in each group, whose anterior descending coronary artery was ligated to make myocardial infarction model. G-CSFs were given for continual 7 days in experimental groups. Cardiac functions were tested by echocardiography one day before and 24 hours and 4 weeks after the modeling of myocardial infarction. The rabbits were sacrificed 4 weeks later for histopathological analysis of the size of myocardial infarction and fibrosis,as well as capillary densities. Results In experimental group, the cardiac functions of ejection fraction （EF）, fractional shortening（FS）, left ventricular wall extent（LVWE）, end-systolic thickness of left ventricular wall （LVWTs） were better than those in the control group at 4 weeks. Ejection fraction increased by 12.89% （being 63.40% in experimental group,while 50.41% in control group）. HE staining showed the structures of infarction regions were deranged in control group; but in experimental group cell were in order, the infracted focus was smaller than that of control group, meanwhilethere were 30% more neogenesis capillaries and less blue fibers stained by Masson staining, the size of infraction was decreased by 30.2%, and the density of capillaries was increased by 30.7%. Conclusions Mobilized G-CSF bone marrow stem cells are effective in treating myocardial infarction through increasing the density of capillaries in the infracted areas and surroundings, diminishing the size of infarction, decreasing collagen fibers, stabilizing cardiac structure and improving the heart function.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第4期363-366,共4页
Chinese Jouranl of Endemiology
基金
黑龙江省科技攻关课题资助项目(GC04C30103)
黑龙江省教育厅海外留学回国人员基金资助项目(2005-74817256)
关键词
粒细胞集落刺激因子
骨髓干细胞
心肌梗塞
心肌结构
Granulocyte colony-stimulating factor
Bone marrow stem cell
Myocardial infarction
Cardiac structure
