摘要
目的：观察肾阴虚状态下豚鼠内耳形态学的变化。方法：选用健康豚鼠24只，随机分为A、B、c3组，每组8只。A组每日肌注醋酸强的松龙注射液50mg／kg，同时用生理盐水灌胃，共10天。B组每日肌注醋酸强的松龙剂量同A组，同时用六味地黄汤灌胃，共10天。C组每日肌注生理盐水，同时用生理盐水灌胃，共10天。观察指标为动物的一般状况，cAMP、cGMP和血浆皮质醇含量，并计算出cAMP／cGMP的比值，在光镜下观察毛细胞形态进行计数。结果：各组内毛细胞几乎无坏死。C组耳蜗基底膜第1—4回三排外毛细胞无明显坏死；A、B组第1—3回外毛细胞均不同程度坏死，尤以第1回（底回）最严重，顶回外毛细胞则基本正常，B组外毛细胞坏死程度要比A组轻（P＜0、05）；A、B组1—3回外毛细胞坏死数分别与c组比较有显著性差异（P＜0．01）。结论：肾阴虚状态下豚鼠内耳毛细胞受到损害，滋补肾阴的中药可保护内耳免受这种损害，进一步证明了中医肾与耳关系论述的科学性。
Objective：To observe the inner ear＇s morphological changes of guinea pigs with kidney -yin -deficiency syndrome. Methods ： Twenty - four healthy guinea pigs were randomized into three groups ： The guinea pigs of group A were intramuscularly injected with prednisolone acetate for 10 days, meanwhile,feeded with physiological saline. Group B which were established models by the same method, were treated with Liuweidihuang Decoction. Group C were intramuscularly injected by physiological saline instead of prednislolne, and the other procedures were the same as group A. The observation indices were：animals general conditions, the contents of cAMP, cGMP and plasma hydrocortisone, the ratil of cAMP/cGMP, calculating the number of cochlear hair cells with the microscope. Results：The inner hair cells of every group animals were nearly not necrosic. The outer hair cells （OHCs） of 1 -4th turn of group C were not distinctly necrosic. The outer hair cells of 1 -3rd turn of group A and B were both necrosic partly, and which of basal turn were especially obvious, but the OHCs of the apical turn were almost normal. The degree of the OHCs of group B was lighter than group A （ P 〈 0.05 ）. The number of necrosic OHCs of 1 - 3rd turn of group A and B was much more than that of group A individually （ P 〈 0.01 ）. Conclusion. The lesions of cochlear hair cells of guinea pigs would be happened when they were in the state of kidney - yin - deficiency syndrome. Herbs of nourishing kidney - yin could protect the inner ear from suffering the hurt. It furtherly proved to be true that the relationship between kidney and ear is very close.
出处
《辽宁中医药大学学报》
CAS
2006年第4期135-137,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
肾阴虚
内耳形态学
疾病模型
动物
豚鼠
kidney - yin - deficiency
morphology of the inner ear
disease models
animal
guinea pigs
