摘要
本文在(25±2)℃和(70±5)％RH条件下,用2 mg·L-1的磷化氢(PH3)气体处理玉米象Sito- philus zeamais Motschulsky,米象Sitophilus oryzae(Linnaeus),谷蠹Rhyzopertha dominica Fbricius,赤拟谷盗Tri- bolium castaneum(Herbst),锯谷盗Oryzaephilus surinamensis(Linnaeus),无色书虱Liposcelis decolor (Pearman)和锈赤扁谷盗Cryptolestes ferrugineus(Stephens)7种储粮害虫的16个品系的成虫,观察记录了所有成虫试虫被PH3击倒的症状,测定各个品系9头试虫的第1、5、9头被击倒的时间,根据中位数理论,将某品系试虫的中间个体(9头中的第5头)被击倒的时间,定义为击倒中时间,英文为Konehdown Time of Median,记为KDTm,用于代表测定品系对PH3的抗性程度。研究结果表明,8个敏感品系的KDTm值均小于(14．3±3．3) min;而8个抗性品系的KDTm值最小(403．7±79．2)min,最大(7 004．8±76．9)min,抗性品系的KDTm明显比敏感品系的长,与FAO方法测定结果相比,KDTm与LC50之间有明显的正相关性,两种方法得出的抗性系数趋势相同,结果具有一致性。据此,建立一种PH3抗性快速测定方法,设立抗性品系判别标准,即KDTm> 30min的品系为PH3抗性品系,KDTm≤30min的品系为敏感品系。该方法的具体操作就是:用2mg·L-1的 PH3处理从粮仓中采集的同种害虫9头活成虫,观察KDTm,即可判断害虫的PH3抗性,为防治该害虫提供重要的决策依据。
The adults of 16 strains in Sitophilus zeamais Motschulsky, Sitophilus oryzae （Linnaeus）, Rhyzopertha dominica Fbricius, Tribolium castaneum （ Herbst）, Oryzaephilus surinamensis （ Linnaeus）, Liposcelis decolor （ Pearman）, Cryptolestes ferrugineus （Stephens） were treated with 2.0mg L^- 1 phosphine （ PH3 ） at （ 25 ± 2℃ ） and 70% ＋ 5% RH. The symptom of knockdown adults in the treatment with PH3was observed and described. The knockdown time of the 1^st, 5^th and 9^th adult had recorded in each test with 9 adults. Based on the median definition, the knockdown time of median （KDTm）, the 5^th adult, was defined as a parameter to indicate the PH3 resistance of a strain, and the resistant factors of the strain can derive from KDTm. The results indicate that the KDTm. of 8 susceptible strains is less than （ 14.3 ± 3.3 ） min. The least one is （403.7 ± 79.2） min, and the top one is （7004.8 ± 76.9） min among all KDTm of the 8 resistant strains. Comparing with result of FAO recommendation methods, KDTm has a close positive relationship with LC50, and the Rf values from the two methods keep a same uptrend. The results to distinguish resistant strains with the two methods are similar. A standard to distinguish resistant strain to PH3 is recommended as follows ： a strain with KDTm higher than 30 min is defined as resistant strain to PH3 ; a strain with KDTm less than 30 min is defined as susceptible strain. Thus, testing 9 adult insects with PH3 of 2mg L^- 1 for one strain in a bulk of stored grain, recording the KDTm, the resistance to PH3 of the strain can be estimated. This quick method to measure PH3 resistance is significant in IPM of grain storage practice.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2006年第3期184-188,共5页
Journal of the Chinese Cereals and Oils Association
基金
中国国家粮食局和澳大利亚国际农业研究中心合作项目(ACIAR PHT98137)河南省科技攻关项目(001020210)
关键词
磷化氢
抗药性
击倒症状
储粮害虫
击倒时间
phosphine （ PH3 ）, resistance, knockdown symptom, knockdown time, stored grain insects
