摘要
利用酸水解把奶粉中的唾液酸释放出来，通过聚丙烯酰胺凝胶除去奶粉中大量乳糖的干扰。利用Cu^2＋和Cr^3＋协同催化唾液酸与间苯二酚的显色，用乙酸丁酯-正丁醇萃取有色物质，在610nm处测定牛奶中唾液酸含量。方法的线性范围0～55．90mg／L，回收率为94％～96％。
Based on the synergistical action of Cu^2＋ and Cr^3＋ to catalyze the color reaction of sialic acid with resorcinol, a spectrophotometric method for the determination of sialic acid in milk powder was developed. The sialic acid was released from the milk powder through acid hydrolysis, and the lactose matrix was removed by using the polyacrylamide gel column. The extracted colored compound was measured by a visible spectrophotometer at the wavelength of 610 nm. The linear range of the method was 0 -55.90 mg/L, and the recovery was 94% -96% .
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第4期129-131,共3页
Journal of Instrumental Analysis
关键词
唾液酸
分光光度法
聚丙烯酰胺凝胶
奶粉
Sialic acid
Spectrophotometry
Polyacrylamide gel
Milk powder
