摘要
目的建立银黄胶囊中黄芩苷和绿原酸含量的高效液相色谱（HPLC）测定法。方法采用Hypersil ODS Agilent（4．6mm×250mm，5μm）色谱枉，甲醇-0．1％磷酸溶液为流动相（梯度洗脱）流速1ml／min；检测波长为318nm。结果绿原酸在0．1004—1．506μg范围内呈良好的线性关系。回归方程l，=15．1149＋2844．8547x，r=0．9999；黄芩苷在0．4120—6．18μg范围内线性关系良好。回归方程l，：34．8131＋2190．0587x，r=0．9999（n=5）。结论该分析方法简便准确，稳定可靠，重现性好，可用于银黄胶囊的质量控制。
Objective To establish the HPLC assay for determination of chlorogenic acid and baicalin in Yinhuang capsule. Methods The chromatographic column of Hypersil ODS Agilent（4.6mm × 250mm ,5μm） was used. The mobile phase for analysis was consisted of methanol-0.1% phosphoric acid solution （gradient elution）, the rate of flow was 1 mL/min, and the detective wavelength was at 318nm. Results The calibration curve of chlorogenic acid presented the favourable linear correlation over the range of 0. 100 4 - 1. 506 μg,and regression equation was Y= 15. 114 9 ＋2 844.854 7X,r =0.999 9. The calibration curve of baicalin presented the favourable linear correlation over the range of 0. 412 0 - 6. 18 p.g, and regression equation was Y = 34. 813 1 ＋ 2 190.058 7X, r = 0. 999 9 （ n = 5 ）. Conclusion The analytical method is convenient, accurate, stable, reliable, better reproducible, and it can be used for the quality control of Yinhuang Capsule.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第7期1195-1196,共2页
Lishizhen Medicine and Materia Medica Research
