摘要
目的探讨腺瘤、大肠癌组织与APC基因突变的关系。方法41例大肠癌与腺瘤组织标本,采用银染单链构相多态性技术分析APC基因突变情况。结果35例原发性结、直肠癌组织有16例发生APC基因突变,突变率为45.7%。8例腺瘤组织检出突变3例,突变率为37.5%。APC基因在散发性大肠腺瘤与大肠癌中均有较高突变频率,两者差异无统计学意义。结果还表明,APC基因突变与年龄、性别、肿瘤性质、位置、类型、分期、淋巴结转移无关。结论APC基因突变在大肠腺瘤阶段就出现,属于大肠癌发生的早期阶段。检测APC基因突变能预测腺瘤的癌变倾向,有助于早期发现大肠癌。
Objective To investigate into the relations of APC gene mutations with colorectal cancer and adenomases. Methods Forty-one colorectal cancer and adenomases cases were collected,then use polymerase chain reaction single strand conformation polymorphism to detect APC gene mutation. Result The positive rates in 35 tumor tissues and 8 adenomases are 45.7%（16135） and 37.5%（3/8） respectively. The total rate is 44%（19/43）. The mutation was not correlated to the age, sex,tumor location,tumor type and dukes period. Conclusion APC gene mutation was found in the adenomas and at early stage of colorectal cancer, so it might be helpful for early discovery of colorectal cancer.
出处
《中国药物与临床》
CAS
2006年第7期499-501,共3页
Chinese Remedies & Clinics
基金
山西省教委基金资助项目(98037)
