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荔枝多酚的分离制备及清除DPPH活性 被引量:26

Preparation of Lichee Polyphenols and Its DPPH Radical Scavenging Activity
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摘要 本文对荔枝壳中多酚类物质的分离制备条件及其清除DPPH活性进行研究。荔枝壳用8倍体积(V/W)50%的丙酮溶液室温下浸提2次,每次浸提2h,回收溶剂,荔枝多酚粗提物得率为34.2%。粗提物经AB-8型树脂柱层析纯化,30%乙醇洗脱组分的多酚回收率为86.8%,纯度为30.0%,该组分清除DPPH的IC50值为4.17μg/ml。 In this paper, the preparation technology of exwacting polyphenols from lichee hulls and its DPPH radical scavenging activity were studied. Lichee hulls were ground, then infused with 8 volumes(V/W) of 50% acetone solution at room temperature for 2 times, 2h per time. Under these conditions, the yield of crude polyphenols was 34.2%. The crude polyphenols was purified with AB-8 type resin, the purity of the 30% ethanol elution part, with the reclaim rate of polyphenols was 85.5%, and its IC50 value on scavenging DPPH radical was 4.17 μ g/ml.
出处 《食品科学》 EI CAS CSCD 北大核心 2006年第7期86-88,共3页 Food Science
关键词 荔枝多酚 制备 二苯基苦基苯肼(DPPH)自由基 lichee polyphenol preparation 2, 2-diphenyl- 1-picrylhydrazyl (DPPH)
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