摘要
目的 观察中药新复方参乌胶囊对β-淀粉样肽前体蛋白（APP）转基因小鼠脑内β-淀粉样肽（A11）代谢及β-和γ-分泌酶的影响。方法 APP转基因小鼠从4～10月龄灌胃给予参乌胶囊。用免疫组织化学法观察APP、Aβ、β位点剪切酶（BACE）和早老蛋白1（PS-1）的表达，并进行图像分析。结果 10月龄APP转基因小鼠模型组与同月龄正常对照组相比，脑内海马区APP、Aβ和PS-1（为γ-分泌酶的催化部位）的表达明显增多，而BACE（即β-分泌酶）的变化不明显。参乌胶囊能明显减少APP转基因小鼠脑内海马区APP的表达，减少脑内All的产生，抑制BACE和PS-1的表达。结论 参乌胶囊抑制脑内All的产生可能是通过减少APP含量及抑制β-和1-分泌酶而起作用的。
Objective To investigate the effects of Chinese herb compound Shen-wu capsule on the metabolism of β-amyloid （Aβ） and β- and γ- seeretase in β-amyloid precursor protein （APP） transgenic mice. Methods APP transgenic mice were administered intragastrically by Shen-wu capsule or water from 4 to 10 months old. The expression of APP, Aβ,β-site APP cleaving enzyme （BACE） and presenilin-1 （ PS-1 ） in hippoearnpus CA1 region was detected by immunohistoehemistry with image analysis. Results Compared with normal group, the expression of APP,Aβ and PS-1 （ the catalytic site of β-secretase） increased obviously in the model group of 10-month-old APP transgenic mice;there was no significant changes in the expression of BACE （ i. e. β-secretase）. Shen-wu capsule decreased the expression of APP,reduced the production of Aβ, and inhibited the expression of BACE and PS-1 in the hippoearnpus of APP transgenic mice. Conclusions Shen-wu capsule could inhibite the production of Aβ through decreasing the content of APP and inhibiting β- and γ-secretase.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第7期924-926,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金项目(30472184)
国家重点基础研究发展计划-"973"项目(2003CB517104)
北京市科技新星计划项目(H020821390190)
首都医学科学发展基金项目(2002-3002)
北京市自然科学基金项目(7982006)
北京市中医药重点学科项目(2005)
