摘要
目的 建立燕窝中4种醛糖的分离分析方法。方法 燕窝中的4种醛糖首先在2mol·L^-1的盐酸甲醇中水解20h，用氢氧化钾-甲醇中和后再在吡啶乙酸酐溶液中乙酰化。采用毛细管气相色谱法，HP-5柱，程序升温法进行测定。结果 甘露糖、半乳糖、N-乙酰半乳糖和N-乙酰葡萄糖在0．0322-0．161，0．316～1．58，0．104～0．520和0．212～1．06mg·L^-1内线性良好；平均回收率分别为95．7％，96．1％，95．1％和97．0％。结论 本方法具有分辨率好、灵敏度高等特点，同时也为真假燕窝的鉴别提供了一种行之有效的方法。
OBJECTIVE To determine the contents of four aldoses in edible bird＇ s nest （EBN）. METHODS Firstly, the sample was hydrolyzed by 2 mol·L^-1 HCl-methanol and neutralized by 2 mol·L^-1 potassium hydroxide, then derivated by acetic anhydride in pyridine. Four aldoses were seperated completely using HP-5 capillary column and programablly increased temperature. RESULTS The linear range of D-Marmot, D-Galactose, N-Acetyl- D-Galactosamine and N-Acetyl- D-Glucosamine were 0.032 2 - 0.161,0.316 - 1.58,0.104 - 0.520 and 0.212 - 1.06 mg·L^-1, respectively. The average recovery was 95.7% ,96.1% ,95.1% and 97.0%, respectively. CONCLUSION A validated GC method is established to determine four aldoses. The method is specific and sensitive.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第14期1108-1110,共3页
Chinese Pharmaceutical Journal
关键词
燕窝
半乳糖
甘露糖
N-乙酰葡萄糖
N-乙酰半乳糖
edible bird＇s nest
D-galactose
D-mannose
N-acetyl- D-galactosamine
N-acetyl- D-glucosamine
