摘要
目的通过细胞内培养和小鼠体内试验观察苦碟子抗呼吸道病毒感染细胞的作用和活体内抗呼吸道病毒感染的效果。方法体外试验采用病毒细胞病变观察(CPE)法测定药物的半数抑制浓度(IC50)和最小有效浓度(MIC)及治疗指数(TI);体内试验采用病毒接种致肺炎动物模型试验,观察肺病变并计算病变率和病变抑制率。结果苦碟子对呼吸道流感病毒H3N2、合胞病毒(RSV)和腺病毒(ADV-3)的治疗指数分别为10,32和16。不同剂量组对3种病毒肺病变抑制率均高于双黄莲对照组(P<0.05)。结论苦碟子对呼吸道流感病毒H3N2、合胞病毒(RSV)和腺病毒(ADV-3)均有良好的抑制作用。
Objective To observe the antagonism against cell infected with respiratory virus of Lxeis Sonchifolia Hance. through cell culture and experiment in mice in vivo. Methods Virus Cytopathugenic Effect （CPE） was used in vitro to measure half inhibitary concentration （ICs0）, inhibitory concentration （MIC） and treat index（TI）. Mouse influenzal viral preumonia experiment was carried out in vivo to observe the pulmonary pathologic change and calculate the pathologic rate and pathologic inhibition rate. Results TI of respiratory influenzal virus H3N2, respiratory syncytial virus （RSV） and adenovirns Ⅲ （ADV-3） was 10, 32 and 16 respectively. The pathologic inhibitive rate in different dosage grops was higher than that of control group （P 〈 0.05）. Conclusion Lxeis Sonchifolia Hance has significant inhibitive effect on RIV- H3N2, RSV and ADV-3.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第8期980-981,共2页
Chinese Journal of Public Health
基金
辽宁省自然基金资助项目(20022151)
关键词
苦碟子
呼吸道病毒
动物试验
Lxeis Sonchifolia Hance
respiratory virus
animal experiment
