摘要
采用温室盆栽方法研究甲基荣莉酸(MeJA)、苯丙噻重氮(BTH)和草酸(OA)诱导油菜对菌核病产生的局部和系统抗性。结果表明,0.01-1.0mmol/LBTH,0.1-1.0mmoL/L MeJA和0.5—15mmol/LOA均能有效诱导局部和系统抗性,系统抗性比局部抗性产生晚。Trypan blue染色结果表明,在MeJA,BTH和低浓度OA诱导过的油菜叶片中.菌丝生长受到抑制,表现为菌丝变粗、弯曲。较低浓度(如0.01mmol/LMeJA,0.5mmol/LOA)下虽然易诱导局部抗性,而系统抗性较弱,0.01mmol/LMeJA几乎不诱导系统抗性。高浓度(如20mmol/L以上)OA有致病作用。
This research showed that methyl jasmonate (MeJA),benzothiadiazole (BTH) and low concentration oxalic acid (OA) can induce resistance to Sclerotinia sclerotiorum in Brassica napus with expression of systemic resistance being later than that of local resistance. In the concentration scale tested, the effect of different MeJA or OA concentrations on induced resistance was not significant, but too low concentration can not induce systemic resistance (e. g. 0.01mmol/L MeJA) and high concentration OA was pathogenic, which caused symptom like that caused by Sclerotinia sclerotiorum. Trypan blue staining showed that the growth of fungal hyphae was abnormal (e. g. thicker and distorted) , which indicated that the mycelia growth was suppressed in induced leaves by MeJA,BTH or low concentration OA.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2006年第2期184-188,共5页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(30070029)
湖北省功能基因组专项(2004BA5068)
中国农科院油料所所长基金
关键词
油菜
菌核病
苯丙噻重氮
甲基荣莉酸
草酸
诱导抗病性
Brasslca napus
Sclerotinia sclerotiorum
Benzothiadiazole
Methyl jasmonate
Oxalic acid
Induced resistance