摘要
目的：采用反相高效液相色谱-蒸发光散射检测法（HPLC—ELSD）同时测定枸橼酸钾中枸橼酸和钾的含量。方法：采用Synergi Fusion-RP80色谱柱（250mm×4．6mm，4μm），以甲醇-水（含甲酸1％）（5：95）为流动相，流速为1mL／min，柱温为室温；ELSD检测条件：漂移管温度为40℃，雾化气体压力为3．5bar。结果：枸橼酸在0．24～11．85μg范围内，进样量与峰面积呈良好的线性关系，r=0．9992，平均回收率为101．9％（n=9），RSD为2．5％（n=6）；钾在0．07～3．61μg范围内，进样量与峰面积呈良好的线性关系，r=0．9994，平均回收率为101．8％（n=9），RSD为1．3％（n：6）。结论：本方法可用于枸橼酸钾中枸橼酸和钾的同时定量测定。
Aim： To simultaneously determine citric acid and potassium in potassium citrate by HPLC-ELSD. Methods： The developed method was based on the separation of citric acid and potassium on Synergi Fusion-RP80 column （250 mm × 4.6 mm, 4 tan） at ambient temperature using methanol-water （containing 1% formic acid） （5：95） as the mobile phase and a flow rate of 1 mL/min; ELSD was used as the detector with nebulizing air pressure of 3.5 bar and the drift tube temperature of 40℃. Results： A linear range of the injected amount of citric acid between 0.24μg and 11.85μg over the responses and the mean recovery of 101.9% （n = 9）with RSD of 2.5% （n = 6）were obtained. And it was also found that a linear range of potassium between 0.07 μg and 3.61 μg with the mean recovery of 101.8% （n = 9）with RSD of 1.28% （n = 6）for potassium existed. Conclusion： A simple and accurate method was established for the simultaneous detemlination of potassium and citric acid in potassium citrate and its sustained-release tablets.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2006年第4期330-332,共3页
Journal of China Pharmaceutical University
