摘要
目的探讨大鼠乳腺癌细胞系制作胫骨癌痛模型。方法选择雌性SD大鼠12只，左侧胫骨上段骨髓腔注入3μl MADB-106大鼠乳腺癌细胞（4．8×10^9个／L）。术前及术后22d，隔日大鼠观察机械痛及辐射热痛阈值变化。于术后8、14、22d，将大鼠麻醉后术侧后肢进行X射线摄片和苏木素-伊红（HE）染色观察术后骨质破坏情况。结果12只大鼠术后14～22d比术后的前12d缩爪阈值和辐射热痛相比差异有统计学意义（P〈0．05）；术后22d放射学显示严重的骨破坏，多处骨皮质缺失；术后14d可见肿瘤细胞充填骨髓腔，引起骨小梁广泛破坏；术后22d可见肿瘤细胞穿破骨皮质。侵及周围肌肉及软组织。结论采用MADB-106乳腺癌细胞系制作大鼠胫骨癌痛模型是可取的。
Objective To duplicate rat models of tibial cancer pain with mammary gland carcinoma cell line. Methods 3μ1 MADB- 106 mammary gland carcinoma cells （4.8 × 10^9 cells·μ1） were injeered into medullary cavity of tibia of twelve female rats weighing 180 - 220 g. Mechanical withdrawal threshold and radiant heat threshold of rats＇ hind paws were measured every other day from one day before operation until 22 days later. The structural damage to the tibia was monitored by radiological analysis, and destruction of bone was stained with hematoxylin and eosin, and observed respectively on the 8th, 14th and 22nd day. Results Mechanical pain threshold and radiant heat threshold in the first 12 days of post-operation were significantly different from that after the 14th day （P 〈 0.05）. On the 22th day after operation, X-rays showed that osteoclasia was serious and loss of cortical bone were much. Cavitas medullaris were full of tumor cells, which induced wide damage of bone trabecula on the 14th day after operation; Tumor cells perforated the cortical bone, and invaded peripheral muscles and soft tissues. Conclusion Our studies indicate that the rat model of tibial cancer pain is successfully by using MADB- 106 mammary gland cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第8期1015-1016,共2页
Chinese Journal of Experimental Surgery
基金
广州中医药大学科研创新基金(2005CO50)
关键词
骨癌痛
脊髓
免疫组织化学
Bone cancer pain
Spinal cord
Immunohistochemistry
