摘要
目的:观察黄芩茎叶总黄酮预处理对缺血再灌注大鼠心肌细胞超微结构的保护作用。方法:实验于2005-03/12在承德医学院基础医学研究所和电镜室完成。①雄性Wistar大鼠40只,随机分为5组:黄芩茎叶总黄酮预处理组[0.05,0.1,0.2g/(kg·d)组]、缺血再灌注组和假手术组。黄芩茎叶总黄酮预处理组于术前1周灌服黄芩茎叶总黄酮[0.05,0.1,0.2g/(kg·d)],缺血再灌注组和假手术组给等量生理盐水。②黄芩茎叶总黄酮预处理组和缺血再灌注组制备缺血再灌注模型,取缺血区、缺血边缘区和非缺血区心肌组织,制备电镜样品并进行超微结构观察。结果:40只大鼠全部进入结果分析。黄芩茎叶总黄酮预处理0.05g/(kg·d)组缺血区心肌细胞肿胀,肌原纤维排列不规则,可见肌溶灶,线粒体肿胀,外膜局部破裂,有的线粒体嵴溶解成空泡状,肌浆网扩张明显。缺血边缘区心肌细胞轻度肿胀,肌膜褶皱,肌浆水肿,肌原纤维较规则,肌溶灶较少,线粒体轻度肿胀,外膜完整,嵴呈波浪形,肌浆网轻度扩张,可见凋亡的心肌细胞。非缺血区部分心肌细胞轻度肿胀,超微结构改变轻微。0.1,0.2g/(kg·d)两组各区心肌细胞损伤程度无明显差别,均较0.05g/(kg·d)组轻微。结论:黄芩茎叶总黄酮预处理对缺血再灌注的心肌细胞超微结构具有保护作用,其机制可能是通过黄芩茎叶总黄酮稳定心肌细胞线粒体、肌原纤维和膜结构来实现的。
AIM： To observe the protective effects of scutellaria baicalensis stem-leaf total flavonoid （SSTF） preconditioning on myocardial ultrastructures of rats with ischemic reperfusion. METHODS： The experiment was conducted in the Institute of Basic Medicine and Electron Microscope Room of Chengde Medical College from March to December 2005. ①Totally＇40 male Wistar rats were selected and randomly divided into 5 groups： SSTF pretreatment groups （0.05, 0.1 and 0.2 g/kg per day groups）, ischemic reperfusion group and sham-operation group. One week before operation, the rats in the SSTF pretreatment groups were infused with different doses of SSTF （0.05, 0.1 and 0.2 g/kg per day）, and the rats in other two groups were infused with normal saline instead. ②The animal models of ischemic reperfusion were established in the SSTF pretreatment groups and ischemic reperfusion group, the myocardial ultrastructural changes of ischemic area, the border ischemic area and non-ischemic area were observed by the transmission electron microscope.
RESULTS： All the 40 rats were involved in the result analysis. In the ischemia area of the 0.05 g/kg per day SSTF group, cardiomyocytes were oncotic, the myofibril arrang6d in irregular way, the dissolved focus of cardiocyte appeared, mitochondria became swelling, their outer membrane naptured locally, while some of crests of mitochondria dissolved into vacuole, sarcoplasmic reticulum expanded obviously. In the border ischemia area, cardiomyocytes were in slightly edematization, epimysium became wrinkled, the sarcoplasma was in oedema, but the myofibril arranged regularly, the dissolved focus of cardiocyte rarely existed, the mitochondria swelled slightly, while their outer membrane was intact, and their crests became undee, sacroplasmic reticulum slightly expanded, the apoptosis in myofibril was presented. In non-ischemia area a portion of myofibril was swelled slightly and their ultrastructural changes in cardiocyte Was also slightly. There was no significant difference in injured degree between 0.1 and 0.2 g/kg per day SSTF groups, both changes were slighter than the 0.05 g/kg per day SSTF group.
CONCLUSION： SSTF preconditioning has protective effects on cardiocyte ultrastructure after ischemic reperfusion by stabilizing mitochondrion, myofibril and cardiocyte membrane.
出处
《中国临床康复》
CSCD
北大核心
2006年第31期49-51,共3页
Chinese Journal of Clinical Rehabilitation
