摘要
本实验选用体重为200～240g 健康 SD 大鼠60只,雌雄参半随机分组,用胆汁酸联合无水乙醇经口灌服制备慢性胃炎的实验模型,并在此基础上观察研究了病理组织学的动态变化,采用多指标同步测试的方法进行实验。病理组织学用 H.E 染色、PAS—AB 染色及 V.G 染色,并以炎细胞浸润程度和粘膜肌层厚度作半定量观察。结果表明:其病变特征与人类慢性胃炎甚为相似,上皮细胞随病程进展,逐渐出现变性坏死、脱落、上皮层缺损;腺体萎缩或囊状扩张。诱发后各组的窦、体部炎细胞浸润显著重于正常组(P 均<0.01),炎症程度以3个月为最重。各组的体部粘膜及1,2,6月组的窦部粘膜明显厚于正常组(P 均<0.01),各组窦、体的粘膜肌层厚度较正常组显著增加(P<0.01),而以6月组为最厚。本模型相当于临床的浅表性和浅表—萎缩性胃炎、其病因学与人类胆汁返流性胃炎相似。用本法复制的模型简便可靠,一般的实验室就能应用,可用于慢性胃炎的病因学、发病学及实验治疗学等研究。
60 healthy Sprague-Dawley rats with half males and half females weighting 200—240g were randomly divided into five groups.The chronic gastritis models were induced with cholic acid and ethanol poured into stomach.The changings of developments of histo-pathological characteristics were observed on many indexes,histological sections with H.E.,PAS—AB,V.G.stain.The inflammative degree and the thickness of muscularis mucosae were measured with a semiquantitative method.The results showed:the pathological feature of models was
similar to that of human chronic gastritis;epithelial cells gradually degenerated,necrosed,and falled off the gastric mucosa with advancing of the disease;epithelium was imperfect;gastric glands atrophyed or dilated as cyst;the inflammative degree of antrum in every model group was much higher than that of normal group(P<0.01);inflammation of 3 months group was the most severe;mucosal thickness of gastric body of every model group and antral mucosal thickness of models of 1 month,two months,6 months were much thicker than that of normal group (P<0.01);muscularis mucosae of 6 months models was the thickest;the models was coresponded to clinical superficial gastritis and superficial-atrophic gastritis;its etiology was similar to that of bile reflux gastritis.This induced method of chronic gastritis is simple,reliable,and may be carried on at any laboratory.The models could be used to study the etiology,pathogenesis,and experimental therapies of.
关键词
胃炎
胆汁返流
实验模型
experimental chronic gastritis
bile reflux
animal model
