摘要
目的建立分离、测定抱茎苦荬菜药材中木犀草素-7-O-β-D-葡萄糖苷的方法。方法采用醇提取、氯仿萃取与硅胶柱分离等手段来分离木犀草素-7-O-β-D-葡萄糖苷；采用RP-HPLC法进行含量测定，色谱条件为：Diamonsin C18（250mm×4．6mm，5μm）色谱柱，以质量分数为0．2％的磷酸溶液-甲醇（体积比为67：33）为流动相，检测波长为260nm，流速为0．8mL·min^-1。结果木犀草素-7-O-β-D-葡萄糖苷质量浓度在4．80-24．0mg·L^-1内线性关系良好（r=0．9997，n=5），平均回收率为99．4％，相对标准差（RSD）为0．97％。结论本法适合测定抱茎苦荬菜药材中木犀草素-7-O-β-D-葡萄糖苷的含量。
Objective To develop a RP-HPLC method for the determination of the content of luteolin-7-O-β-D-D-glucopyranoside in Ixeris sonchifolia （Bge.）Hance. Methods The luteolin-7-O-β-D-glucopyranoside was obtained by the extraction with 70 % EtOH and precipitated by the method of column chromatography. It was separated on a Diamonsin C18 （4.6 mm × 250 mm, 5 μm） column with CH3OH-H2O（0.2 96 H3 PO4） （33 ： 67）as the mobile phase, The UV detective wavelength was set at 260 nm, the flow rate was 0.8 ml/min. Results The average recovery is 99.4 %, and RSD is 0.97 %. The linear calibration curves are obtained in the ranges of 4.8-24.0 mg·L^-1. Conclusions The method is simple and accurate. It can be applied to the determination of the concentration of the luteolin-7-O-β-D-glucopyranoside.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2006年第8期518-520,共3页
Journal of Shenyang Pharmaceutical University
