摘要
目的通过观察不同年龄正常皮肤（NS）、增生性瘢痕（HTS）的转化生长因子β1（TGF-β1）表达与胶原构成，探讨正常皮肤胶原构成与年龄的相关性和不同年龄瘢痕易发性。方法采用SABC免疫组化方法观察NS各年龄组：胎儿、1～19岁（儿童青少年组）、20～50岁（成年组）、）60岁（老年组）和HTS组：1～19岁、20～50岁的TGF-β1表达以及Ⅰ、Ⅲ型胶原的含量和比例。结果①HTS组的TGF-β1含量显著大于正常皮肤。1～19岁HTS组TGF-β1表达较20～50岁组的增高具有显著性差异（P〈0．01）。胎儿组皮肤TGF-β1表达低于其余组正常皮肤（P〈0．01）；②除1～19岁组与20～50岁组之间的Ⅰ型胶原无差异外，其余各组之间的差异均有显著性意义。）60岁组的Ⅲ型胶原明显低于其他年龄组。正常皮肤Ⅰ／Ⅲ型胶原比例随年龄增加呈上升趋势。HTS组织的Ⅰ／Ⅲ型胶原比例明显高于正常皮肤（NS），在1～19岁HTS组中增高更为显著。结论Ⅰ、Ⅲ型胶原的构成和比例决定正常皮肤的弹性、厚度和张力。TGF-β1表达增高促使增生性瘢痕的形成，1～19岁（儿童青少年时段）HTS组中TGF-β1含量显著增高，导致Ⅰ／Ⅲ型胶原比例失调，可能是该年龄段增生性瘢痕发生率较高的原因之一。
Objective To investigate the relationship of expression of TGF-β1 and collagen in tissues from normal skin and hypertrophic scar in different age periods. Methods The expressions of TGF-β1, type Ⅰ and type Ⅲ collagen of hypertrophic scar in two groups （1 - 19 years old and 20 - 50 years old group） were stained with immunohistochemistry. And the same staining was performed on the normal skin divided into 4 groups-fetal, 1 - 19, 20 - 50 and more than 60 years old. Results The expression of TGF-β1 protein in hypertrophic scar was increased. The expression of TGF-β1 in hypertrophic scar in 1 - 19 years old group was significantly higher than that in 20- 50 years old group （P 〈0. 01 ）. The levels of type Ⅰ collagen in all group of normal skin were significant difference except for that between 1 - 19 year old and 20 - 50 year old group. The type Ⅲ collagen of more than 60 years old group was less than the other groups. The ratio of type Ⅰ/type Ⅲ collagen of normal skin was gradually increased with aged. The ratio of type Ⅰ/type Ⅲ collagen of hypertrophic scar was significantly elevated, and that ratio was higher in 1 - 19 years old group. Conclusions Significantly increased TGF-β1 in hypertrophic scar that stimulates more type Ⅰ collagen synthesizing and enhances the ratio of type Ⅰ and type Ⅲ collagen may be one of the causes of higher incidence of scarring in the period of 1 - 19 year old.
出处
《中华小儿外科杂志》
CSCD
北大核心
2006年第8期393-396,共4页
Chinese Journal of Pediatric Surgery
关键词
皮肤
转化生长因子Β1
胶原
Skin
Transforming growth factor beta1 （TGF-β1）
Collagen
