摘要
报道了一种基于等离子体修饰的PVC膜脲酶生物传感器。应用微波等离子体化学气相沉积技术，在pH敏感PVC膜上修饰一层带有氨基的乙二胺等离子体聚合物薄层，通过戊二醛共价交联固定脲酶分子，制成脲酶传感器。这种乙二胺等离子体聚合物薄层对pH敏感膜的响应特性影响小，且其表面氨基使得pH敏感膜更加易于与H^＋结合，检测范围向高pH方向偏移，响应斜率为45．73mV／pH，比未修饰的pH敏感膜电极的响应斜率42．81mV／pH有所提高。该脲酶传感器对尿素显示了良好的传感性能，其响应时间约为200s；在6．0×10^-4～8．4×10^-3mol／L范围，脲酶传感器的电位响应值与尿素浓度的对数存在良好的线性关系，相关系数为0．9978，检测下限为5．0×10^-5mol／L。
A new urease biosensor based on the plasma-modified PVC membrane has been developed. Using microwave plasma technique, an ethylene diamine plasma-polymerized layer with amine groups was first deposited on the pH-sensitive PVC membrane. The abundant amide groups make the plasma-modified pH- sensitive membrane easier to bind H^＋ , and thus the response range of the pH sensitive membrane has some shift toward high pH value and the response slope of 45. 73 mV/pH is over that of common pH sensitive membrane, 42.81 mV/pH, showing an enhancing pH response. After activated with 2.5% glutaraldehyde solution, urease molecules were covalently immobilized onto the plasma-polymerized pH-sensitive PVC membrane surface and the urease biosensor was then obtained. The urease biosensor has a response time of less than 200 s and a detection limit of 5.0 × 10^-5 mol/L, and there is a good linear relationship between the potential response and the logarithm value of the urea concentration in the range of 6.0 × 10^-4- 8. 4 × 10^-3 mol/L with an interrelated coefficient of 0. 997 8.
出处
《传感技术学报》
CAS
CSCD
北大核心
2006年第4期933-936,共4页
Chinese Journal of Sensors and Actuators
基金
国家自然科学基金资助(20205004)
教育部科学技术研究重点项目资助(106122)
