摘要
采用聚丙烯酰胺凝胶圆盘电泳技术，对云南滇池高背鲫鱼的成熟卵和胚胎不同发育时期的乳酸脱氢酶（Ｌａｃｔａｔｅｄｅｈｙｄｒｏｇｅｎａｓｅ，ＥＣ．１．１．１．２７，ＬＤＨ）同工酶的表达情况进行了分析，结果如下：１．在所研究的整个发育过程中，ＬＤＨ持续表达了４条酶带。这些持续表达的酶带，在发育过程中可能执行最基本的代谢功能。２．随着胚胎发育的进展，从心搏期起，尤其到肝形成期，ＬＤＨ新酶带逐渐增加，这可能是胚胎细胞核中相应基因开始表达的结果，有些也可能是转录后或翻译后有关调节机制开始作用的结果。３．云南滇池高背鲫鱼胚胎发育过程中ＬＤＨ同工酶的表达有一明显的特点，即与近缘的四倍体鲫鱼和鲤鱼相比，ＬＤＨ的酶带数显著增多，滇池高背鲫鱼是进化六倍体，所增加酶带很可能是在进一步多倍化以后的进化过程中，重复基因发生突变的结果。
In the present study. expression of Lactate Dehydrogenase Isozymes (LDH) during embryogensis of Dianchi High-back crucian carp was analysed by polyacrylamide gel disc electrophoresis. The results were as follows: 1) Four isozymes were present at all developmental stages and may function in general metabolism. not relating to differentiation of cells. 2) Some new isozymes appeared at Heartbeat stage and Liver forming stage, respectively. it suggests that the embryonic gene have begun to express. 3) One fundamental difference in expression of LDH isozyme existing between this hexaploidized,gynogenesis fish and its tetraploid relatives was the increse of the number of isozyme bands. The increase of band numbers could perhaps result from expression of the duplicated genes undergone mutation.
出处
《西南农业学报》
CSCD
1996年第4期53-57,共5页
Southwest China Journal of Agricultural Sciences
基金
云南省教委科研基金
关键词
LDH
发育
遗传学
滇池高背鲫
同功酶
LDH isozyme
Developmental genetics
Electrophoresis
Dianchi High-back crucian carp (Carassius auratus)
