摘要
抗菌肽ＬｃＩ基因转化杨树的阶段研究＊李陈颖李铃韩一凡关键词抗菌肽ＬｃＩ基因、转化、杨树杨树（Ｐｏｐｕｌｕｓｓｐｐ．）是“三北”防护林和四旁绿化的主要树种，在防风固沙、农田林网、四旁绿化及水土保持方面起着重大作用［１］。目前，我国林木抗虫抗病主要以化...
Leaves of in vitro Populus deltoides, P. nigra and P. euramericana were used as explant to be transformed with antibacterial gene LcI which was synthesized according to the sequence of antibacterial protein of Bacillus spp. It was demonstrated that the insect mortality of this antibacterial protein to Anoplophora glabripennis was more than 75%. Transformants were selected by 40 mg/L kanamycin, and the rooting percentage was about 80.7% in the rooting media supplemented with 40 mg/L kanamycin. PCR analysis showed that antibacterial gene has been successfully transformed into these popalr chromosomes.
出处
《林业科学研究》
CSCD
北大核心
1996年第6期646-649,共4页
Forest Research
关键词
抗菌肽
LcI基因
转化
杨树
antibacterial gene LcI, transformation, poplar
