摘要
目的探讨红藻氨酸(KA)诱导癫疒间大鼠血清及海马组织中神经元特异性烯醇化酶(NSE)和S100β蛋白(S100β)的变化及其临床意义。方法180只Wistar大鼠随机分为对照组、KA组和卡马西平(CBZ)组,后两组再按癫疒间发作后1h、4h、12h、24h、48h和72h不同时点分为6个亚组。以放射免疫法(RIA)和酶联免疫吸附试验(ELISA)分别测定大鼠血清和海马匀浆液中NSE和S100β的变化。结果癫疒间发作72h内,血清和海马匀浆液中NSE和S100β的含量是一个动态变化过程,且呈同步变化的趋势,在12h时均达到峰值。在4～48h时,KA组和CBZ组的二者含量均明显高于对照组(P<0.05～0.01)。结论癫疒间发作后NSE和S100β的含量升高,二者可作为癫疒间发作后脑组织损伤的一个参考指标。
Objective To explore the change and its clinical significance of neuron-specific enolase （NSE） and S-100β in serum and hippocampus tissue in rats with epilepsy induced by Kainic acid （KA）. Methods 180 Wistar rats were randomly classified into control, KA and carbamazepine （ CBZ ） group, and the later two groups were further divided into 6 sub-groups （ 1 h, 4 h, 12 h, 24 h, 48 h and 72 h ） according to epileptic attack at different time point. The NSE and S-100β concentrations in serum and homogenate of hippocampus were determined by radioimmunity assay （RIA） and enzyme linked immunosorbent assay （ELISA）, respectively. Results The concentrations of NSE and S-100β were dynamic change during 72 h after epileptic attack in serum and hippocampus homogenate, and the changes were synchronous. The concentrations reached peak at 12 h. Both NSE and S-100β concentrations in KA and CBZ group were obviously higher than those in control group （ P 〈 0.05 - 0. 01 ） during 4～48 h. Conclusion Rising up of NSE and S-100β concentrations may be regarded as reference indexes of brain injury after epileptic attack.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第4期287-289,共3页
Journal of Clinical Neurology
基金
兰州军区科学基金资助项目(YN04)
