摘要
目的 建立紫外分光光度法测定补骨脂药材中总呋喃香豆素的含量。方法 补骨脂药材粉末经索氏提取2h，过聚酰胺柱后，采用紫外分光光度法，检测波长为245nm对补骨脂药材中的总呋喃香豆素进行测定。结果 采用该方法可准确测定补骨脂药材中总呋喃香豆素的含量，补骨脂素在1．008～10．08μl／ml范围内吸光度与浓度的线性关系良好，样品平均回收率为98、7％，精密度RSD为0．11％，重复性RSD为2．8％，稳定性RSD为0．75％（4h内）。结论 该方法简单、准确，可用于补骨脂药材中总呋喃香豆素的含量测定。
Objective To establish a UV spectrophotometry for determination of the total furanocoumarins in Fructus Prosraleae. Methods The powder of Fructus Psoraleae was extracted by CH3OH for two hours and puerariaed by polyamide column chromatography ;when it was determined by UV spectrophotometry, wave length was 245 nm. Results The method had good linear relationship from 1. 008μl/ml to 10.08 μl/ml,and the recovery rate was 98.7%. The relative standard deviation of accurate test and stability test were 2.8% and 0.75% respectively. Conclusion The method is simple and accurate, and can be used for determination of the total furanocoumarins in Fructus Psoraleae.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第8期1401-1402,共2页
Lishizhen Medicine and Materia Medica Research
基金
重庆市科委攻关资助项目(No.CSTC
2005AD5020)
