摘要
目的:建立高效液相色谱法测定酸水解后金银花药材中常春藤皂苷元和齐墩果酸的含量。方法:采用反相高效液相色谱法,色谱柱为 HiQ Sil C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(85:15),流速1 mL·min^(-1),柱温为室温,进样量10μL,检测波长210 nm。采用硫酸加热回流水解4 h,氯仿索氏回流提取皂苷元。结果:常春藤皂苷元和齐墩果酸的线性范围分别为1.15～28.90μg及1.02～25.50μg,平均回收率分别为98.9%(RSD=1.8%)及98.5%(RSD=2.1%)。结论:所建立的 HPLC 方法重复性好,专属性强,为金银花药材质量控制标准的建立提供科学依据。
Objective： To establish a method for the determination of hederagenin and oleanolic acid in Flos Lonicerae Japonicae by RP - HPLC. Methods： The analysis was performed on HiQ Sil C18 （250 mm × 4. 6 mm, 5 μm ） column with mobile phase contained acetonitrile - water （ 85 ： 15 ）. The flow rate was 1.0 mL · min^-1. The column temperature was at room temperature and the injected volume was 10 μL. The detected wavelength was set at 210 nm. It was hydrolyzed with sulfuric acid and extracted with chloroform. Results：The linear ranges of hederagenin and oleanolic acid were 1.15 -28.90 μg and 1.02 -25.50 μg,respectively. The average recoveries of hederagenin and oleanolic acid were 98.9% （RSD = 1.8% ）and 98.5 % （RSD = 2. 1% ）, respectively. Conclusion：The developed method is simple, accurate, and specific which can be used for the quality control of Flos Lonicera Japonicae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第7期885-887,共3页
Chinese Journal of Pharmaceutical Analysis
基金
香港 Industrial Support Fund AF/281/97
国家科技部创新药物和中药现代化资助课题
