摘要
以8个籼稻品种作材料，研究了携带抗稻瘟病基因Pi9片段的pCAMBIA1301质粒的根癌农杆菌EHA105转化籼稻幼胚愈伤组织的影响因素。结果表明，NB培养基适用于籼稻愈伤组织的诱导，共培养时间以3d为宜。40mg/L的潮霉素可用于籼稻愈伤组织的筛选，抗性愈伤率在69．4％-85．3％之间；抗性愈伤组织经分化培养，幼苗分化率在15．4％-47．4％；鉴于多数籼稻愈伤组织分化时对潮霉素特别敏感，因此分化培养基中不加潮霉素；但为了减少假阳性，生根培养基中分别用20mg／L和30mg／L的潮霉素对幼苗进行筛选。
Factors influencing the frequency of rice transformation mediated by A. tumefacience strain EHA105 harboring pCAMBIA1301 plasmid carrying blast-resistant Pi9 gene were investigated on 8 lndica rice cultivars. The result showed that NB medium was suitable for calli induction of lndica rice, the best time for the co-culture of callus and Agrobacterium was 3 days, 40mg/L hygromycin was fit for resistant selection, 69.4% - 85.3% resistant calli survived. In differentiation medium, the frequencies of eight lndica rice varieties were between 15.4% - 47.4%. Most of lndica rice calli were sensitive to hygromycin in differention medium, hygromycin was unfit for differention. In order to reduce false positive plants, we used 20mg/L and 30mg/L hygromycin to select the young green plants.
出处
《核农学报》
CAS
CSCD
北大核心
2006年第4期263-268,共6页
Journal of Nuclear Agricultural Sciences
基金
国家863项目(2005AA241010)
国家自然科学资金资助项目(30571063
30470990)
湖南农业大学芙蓉学者计划资助项目
关键词
籼稻
抗性愈伤
分化
潮霉素
Indica rice
resistant calli
differentiation
hygromycin
