摘要
目的:观察东莨菪碱(scopolamin,Sco)对吗啡(morphine,Mor)致小鼠依赖性作用的影响及其机制分析。方法:连续7 d腹腔内注射吗啡建立吗啡依赖小鼠模型;热板法测定痛阈;纳络酮(naloxone,Nal)催促实验计数小鼠跳跃次数和跳跃动物率;流式细胞术测量海马细胞内游离Ca2+([Ca2+]i)浓度。结果:吗啡依赖小鼠的痛阈明显下降,跳跃次数和跳跃动物率明显增加,海马[Ca2+]i明显增加。给予东莨菪碱(4mg.kg-1×7 d)后,吗啡依赖小鼠海马[Ca2+]i明显减少(P<0.05),痛阈提高(P<0.01),跳跃次数和跳跃动物率减少(P<0.05)。结论:东莨菪碱具有对抗吗啡依赖性的作用,其机制可能与降低脑细胞内游离Ca2+水平有关。
Aim： To observe the effect and mechanism of scopolamin on morphine（Mor）-induced mice dependence. Methods： The Mor-dependent mice model was established by intraperitoneal（i p） administered Mor for seven days. Pain threshold, times of jump and hippoeampus intracellular free calcium ion concentration（ [ Ca^2 ＋ ]i） were determined by the heat plate test, naloxone（ Nal）-precipitated jumping response and flowcytometry, respectively. Results： The pain threshold of Mor-dependent mice decreased significantly while there was a marked increase in times of jump, the rate of jumping animals and hippoeampus[ Ca^2＋ ]i. Co-administered scopolamin, the pain threshold of Mor-dependent mice increased significantly; the number of jump, the rate of jumping animals and hippocampus [ Ca^2 ＋ ]i all decreased significantly. Conclusion： Scopolamin could antagonize the Mor-induced mice dependence, which could be related to decreasing the levels of brain intracellular free calcium.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2006年第3期307-309,共3页
Chinese Journal of Applied Physiology
基金
河北省卫生厅资助项目(0325)
关键词
东莨菪碱
吗啡
海马
痛阚
纳络酮跳台反应
[CA^2+]I
scopolamin
morphine
pain threshold
Nal-precipitated jumping response
intracellular calcium
hippocampus
